- Reliable - fully validated in-house with human, mouse, and rat cell lysates and Western blot correlation
- Sensitive - quantify less than 33.8 pg/ml β-catenin
- Simple - liquid color-coded reagents reduce error
The β-Catenin EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours that measures pan β-Catenin. Absorbance is read at 450 nm. This kit has been validated in-house with human, mouse, and rat cell lysates and correlated Western blot. The β-Catenin EIA kit detects as little 33.8 pg/mL β-catenin surpassing semi-quantitative Western blot analysis and provides simple liquid color-coded reagents that reduce laboratory error.
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Product Details
| Alternative Name: | Cadherin-associated protein |
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| Sensitivity: | 33.8 pg/ml (range 125 - 8,000 pg/ml) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of β-Catenin in cell lysates of human, mouse, and rat origin. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human, Mouse, Rat
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| Use/Stability: | Store all components at 4°C, except standard at -20°C. |
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| Shipping: | Dry Ice and Blue Ice |
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| Contents: | Microtiter plate, Conjugate, Antibody, Assay buffer 13, RIPA Cell lysis buffer 2, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2 |
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| Scientific Background: | β-catenin is a 781 amino acid adherens junction protein that plays an important role in two unrelated cellular functions. Adherens junctions are critical for establishment and maintenance of epithelial layers, such as those found lining the surface of organs. Involved in both cell-cell adhesion and in the wnt/wg signaling pathway, β-catenin can be found in the cytoplasm or the nucleus. In the cytoplasm, β-catenin is unstabilized by phosphorylation and association with E-cadherin. When β-catenin translocates to the nucleus, it has comparatively low levels of phosphorylation. Wnt/wg signaling results in β-catenin accumulation and transcriptional activation of specific target genes during development. Deregulation of β-catenin signaling is an important event in the evolution a number of malignancies, including colon cancer, melanoma, ovarian cancer, and prostate cancer. The homology between human, mouse and rat β-catenin is 99.9%. |
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| UniProt ID: | P35222 (human), Q02248 (mouse), Q9WU82 (rat) |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Secreted frizzled-related protein-2 inhibits doxorubicin-induced apoptosis mediated through the Akt-mTOR pathway in soleus muscle: H. Merino, et al.; Oxid. Med. Cell. Longev.
2018, 6043064 (2018),
Application(s): ELISA using mouse soleus muscle,
Abstract;
Full Text
Activating transcription factor-3 induction is involved in the anti-inflammatory action of berberine in RAW264.7 murine macrophages: Y.A. Bae, et al.; Korean J. Physiol. Pharmacol.
20, 415 (2016),
Application(s): ELISA (to find proinflammatory cytokine production),
Abstract;
Full Text
Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug: M.A. Ali, et al.; PLoS One
10, e0134648 (2015),
Application(s): ELISA using rat tissue homogenate,
Abstract;
Full Text
Effect of dietary caraway essential oils on expression of β-catenin during 1,2-dimethylhydrazine-induced colonic carcinogenesis: A. Allameh, et al.; J Nat Med.
67, 690 (2013),
Application(s): EIA using rat colon tissue extracts,
Abstract;
Early activation of the beta-catenin pathway in osteocytes is mediated by nitric oxide, phosphatidyl inositol-3 kinase/Akt, and focal adhesion kinase: A. Santos, et al.; BBRC
391, 364 (2010),
Application(s): EIA using mouse cell lysate,
Abstract;
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