Replaces Prod. #: ALX-850-056
- Fast - results from up to 39 samples in duplicate in just 3 hours
- Quantitative - fully quantitative results surpass semi-quantitative Western blot analysis
- Easy-to-use - liquid color-coded reagents reduce error
The Bcl-2 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours that measures pan Bcl-2. Absorbance is read at 450 nm. Obtain results from up to 39 samples in duplicate per 96-well kit. The Bcl-2 (human), EIA kit provides fully quantitative results that surpass semi-quantitative Western blot analysis and easy-to-use liquid color-coded reagents that reduce error.
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Product Specification
| Sensitivity: | 3.8 pg/ml (range 18.8 - 1,200 pg/ml) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of Bcl-2 in cell lysates of human origin. |
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| Species reactivity: | Human
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| Use/Stability: | Store all components at 4°C, except standard at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Contents: | Microtiter plate, Conjugate, Antibody, Assay buffer 13, Wash buffer concentrate, Standard, TMB Substrate, Cell lysis buffer 2, Stop solution 2 |
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| Scientific Background: | Bcl-2 (B-cell lymphoma 2) is an oncogenic protein that inhibits apoptosis. It is located within the mitochondrial membrane, endoplasmic reticulum and nuclear envelope. Bcl-2 is encoded by a 230kb gene that results in a 24-26kDa protein4. Bakhshi first identified Bcl-2 as an apoptotic proto-oncogene while studying chromosomal translocations in human follicular lymphomas. At approximately the same time Tsujimoto and Croce made the same observation. The function of Bcl-2 was first described by Vaux when it was demonstrated that it promoted the survival of factor dependent cells. Bcl-2 overexpression has been implicated in a wide variety of malignancies including (but not limited to) breast, prostate, skin, colon and pancreatic cancers. Related anti-apoptotic proteins include Bcl-xL, Mcl-1 and Bcl-w. Pro-apoptotic proteins in the Bcl-2 family include Bax, BAD, Bak, Blk and Bid. |
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| UniProt ID: | P10415 |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Alteration in the Balance of Prosurvival and Proapoptotic Signalling Pathways Leads to Sequence-Dependent Synergism Between Docetaxel and Sorafenib in Human Non-small Cell Lung Cancer Cell Lines: X. He & T. Zhang; Cell Biochem. Biophys.
68, 411 (2014),
Abstract;
Enhancing In Vivo Survival of Adipose-Derived Stromal Cells Through Bcl-2 Overexpression Using a Minicircle Vector: J. Hyun, et al.; Stem Cells Transl. Med.
2, 690 (2013),
Abstract;
Full Text
Opioid-regulated pro- and anti-apoptotic gene expression in cancer cells: K. Gach, et al.; Centr. Eur. J. Biol.
7, 411 (2013),
Abstract;
Optically Traceable Solid Lipid Nanoparticles Loaded with siRNA and Paclitaxel for Synergistic Chemotherapy with In situ Imaging: K. H. Bae, et al.; Adv. Healthc. Mater.
2, 576 (2013),
Abstract;
Anti-tumorigenicity of dietary α-mangostin in an HT-29 colon cell xenograft model and the tissue distribution of xanthones and their phase II metabolites: C. Chitchumroonchokchai, et al.; Mol. Nutr. Food Res.
57, 203 (2012),
Abstract;
Full Text
Ginkgo extract EGb 761® shields from slowly accumulating neurodegenerative-like changes in a newly developed cell culture model induced by the combined action of low doses of antimycin A1 and 2-deoxy-d-glucose: K. Plaschke, et al.; J. Neural Transm.
118, 1247 (2011),
Abstract;
