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IL-1β (rat), ELISA kit

 
ADI-900-131A 96 wells 694.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: ADI-900-131

  • Sensitive - use less precious sample per test
  • Higher throughput - test up to 40 samples in duplicate in less than 4 hours
  • Quantitative - obtain statistically significant results and compare data between multiple experiments
  • Ready-to-use - pre-coated plates save time and reduce errors
The IL-1β (rat), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in < 4 hours.

Product Details

Alternative Name:Interleukin-1β
 
Sensitivity:4 pg/mL (range 31.3 - 2,000 pg/mL)
 
Assay Time:<4 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of IL-1β in culture supernatants and serum of rat origin.
 
Wavelength:450 nm
 
Species reactivity:Rat
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Contents:Microtiter plate, SA-HRP concentrate, SA-HRP dilution buffer, Biotinylated antibody, Standard, Standard diluent, Wash buffer concentrate, TMB Substrate, Stop solution
 
Scientific Background:The interleukin-1 (IL-1) cytokine family consists of IL-1α, IL-1β, and IL-1Rα (receptor antagonist) which are expressed primarily by endothelial cells and activated macrophages. IL-1α and IL-1β elicit pro-inflammatory cytokine production and cellular activation, and are the primary mediators of hypothalamic regulation that results in fever. IL-1α and IL-1βsignals are transmitted via a dual subunit IL-1 receptor composed of IL-1R1 and the IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP is vital to IL-1 signaling, as it recruits the IL-1 receptor-associated kinase (IRAK) to the receptor complex, initiating NF-κB pathway activation.
 
Technical Info/Product Notes:Cited samples:
Cytokine ELISAs Cited Samples
 
UniProt ID:Q63264
 
Regulatory Status:RUO - Research Use Only
 
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

Plate Reader Just Got Personal
 

Product Literature References

Tumor Necrosis Factor-α Mediates Lung Injury in the Early Phase of Endotoxemia: K.Y. Chen, et al.; Pharmaceuticals 15, 287 (2022), Abstract;
Assessment of Anti-Inflammatory and Antioxidant Effects of Citrus unshiu Peel (CUP) Flavonoids on LPS-Stimulated RAW 264.7 Cells: A. Karthikeyan, et al.; Plants 10, 2209 (2021), Abstract;
Involvement of the P2X7 receptor in the migration and metastasis of tamoxifen-resistant breast cancer: effects on small extracellular vesicles production: M. Park, et al.; Sci. Rep. 9, 11587 (2019), Abstract; Full Text
The anti-inflammatory effects of E-α-(p-methoxyphenyl)-2′,3,4,4′-tetramethoxychalcone are mediated via HO-1 induction: K.B. Kaufmann, et al.; Int. Immunopharmacol. 35, 99 (2016), Application(s): Cell media supernatant of mouse RAW264.7 macrophage culture, Abstract;
Hydrogen sulfide protects against amyloid beta-peptide induced neuronal injury via attenuating inflammatory responses in a rat model: H. Fan, et al.; J. Biomed. Res. 27, 296 (2013), Application(s): ELISA using rat hippocampus, Abstract;

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