- Convenient - pre-coated plates and liquid color-coded reagents reduce errors
- Quantitative - save time and achieve fully quantitative results compared to semi-quantitative Western blot analysis
- Specific - no reactivity with structurally related molecules
The XIAP (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. Save time, money, and precious sample with fully quantitative results and increased sensitivity compared to Western blot analysis.
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Product Specification
| Alternative Name: | X-linked inhibitor of apoptosis |
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| Sensitivity: | 90.6 pg/ml (range 312.5 - 10,000 pg/ml) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of human XIAP in cell lysates. |
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| Species reactivity: | Human
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| Crossreactivity: | Human XIAP (100%), Survivin (3.2%) and <0.1%: cIAP-1, Caspase-3, Smac, DIABLO |
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| Use/Stability: | Store all components at 4°C, except standard at -20°C. |
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| Shipping: | Shipped on Blue Ice |
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| Contents: | Microtiter plate, Conjugate, Antibody, Assay buffer 21, RIPA Cell lysis buffer 2, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2 |
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| Scientific Background: | XIAP (X-linked inhibitor of apoptosis) belongs to the IAP (inhibitors of apoptosis) family, which is characterized by one or more BIR (baculovirus IAP repeat) domains. These proteins have multiple biological functions that include binding and inhibiting caspases, regulating cell cycle progression, and modulating receptor-mediated signal transduction. XIAP functions by direct binding and inhibition of several caspases, including caspase-3, caspase-7 and caspase-9. |
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| UniProt ID: | P98170 |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
The flavonolignan silibinin potentiates TRAIL-induced apoptosis in human colon adenocarcinoma and in derived TRAIL-resistant metastatic cells: H. Kauntz, et al.; Apoptosis
17, 797 (2012),
Application(s): ELISA using cell lysates,
Abstract;
