13(S)-HODE ELISA kit



ADI-900-108	96 wells	491.00 USD
Do you need bulk/larger quantities?

Specific - negligible reactivity with related molecules
Simple- ready-to-use liquid color-coded reagents reduce error
Trusted - cited in peer-reviewed publications
Reliable - reproducible results day-after-day and lot-after-lot

The 13(S)-HODE EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 4 hours. Absorbance is read at 405 nm. The kit provides ready-to-use non-radioactive liquid color-coded reagents that reduce error in the lab.

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Product Details

Alternative Name:	13-hydroxyoctadecadienoic acid

Sensitivity:	1.6 ng/ml (range 3.9 - 1,000 ng/ml)

Assay Time:	4 hours

Applications:	ELISA, Colorimetric detection

Application Notes:	For the quantitative determination of 13(S)-HODE in culture supernatants, cell lysates, serum, saliva, tissue, and urine from any species. Cited sample type includes plasma (ref. 1).

Wavelength:	405 nm

Species reactivity:	Species independent

Use/Stability:	Store all components at 4°C, except conjugate -20°C.

Shipping:	Shipped on Blue Ice

Contents:	GxR IgG Microtiter plate, Assay buffer, Conjugate, Antibody, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution

Scientific Background:	The major human dietary poly-unsaturated fatty acid is linoleic acid, an essential fatty acid that can be metabolized to either 13-hydroxyoctadecadienoic acid [13(S)-HODE] via lipoxygenase or [13(R)-HODE] by p450 enzymes. Alternatively, cyclooxygenase metabolism of linoleic acid results in the production of hydroxyeicosatetraenoic acid [15(S)-HETE]. Changes in the intra-cellular ratio of 13(S)-HODE to 15(S)-HETE have been linked to a number of physiological conditions including cancer and cardiovascular disease. 13(S)-HODE helps to mediate cell adhesion and excessive proliferation that is critical in the formation of tumors and metastases, as well as the formation of atherogenic plaques.

Regulatory Status:	RUO - Research Use Only

Compatibility:	This product is compatible with the Absorbance 96 Plate Reader.

Product Literature References

Increased 15-lipoxygenase-1 expression in chondrocytes contributes to the pathogenesis of osteoarthritis: K. Chen, et al.; Cell Death Dis. 8, e3109 (2017), Application(s): Chondrocyte cell lysate, Abstract;

The oxidative stress mediator 4-hydroxynonenal is an intracellular agonist of the nuclear receptor peroxisome proliferator-activated receptor-β/δ (PPARβ/δ): K. Prabhu, et al. ; Free Radic. Biol. Med. 42, 1155 (2007), Application(s): EIA using human plasma (ref. 1), Abstract;

The transcription factor GATA-6 is overexpressed in vivo and contributes to silencing 15-LOX-1 in vitro in human colon cancer: I. Shureiqi, et al. ; FASEB J. 21, 743 (2007), Application(s): EIA using human cell lysates & culture supernatants, Abstract;