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TNF-α (rat), ELISA kit

ADI-900-086A 96 wells 638.00 USD
Do you need bulk/larger quantities?
  • Sensitive - detect <30 pg/ml rat TNFα
  • Fast - fully quantitative results in just 3 hours
  • Easy-to-use - liquid color-coded reagents reduce errors
  • Save time, money, and precious sample compared to Western blot analysis
The TNF-α (rat), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm.
TNF-&alpha; (rat), ELISA kit Standard curve
TNF-&alpha; (rat), ELISA kit Standard curve
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TNF-&alpha; (rat), ELISA kit Standard curve TNF-&alpha; (rat), ELISA kit Standard curve

Product Details

Alternative Name:Tumor necrosis factor-α, TNFSF 2
Sensitivity:12.0 pg/mL in Assay Buffer 28; 26.7 pg/mL in non-conditioned 10% FBS culture media (diluted 1:8 into Assay Buffer 28) (range 31.3 - 2000 pg/mL)
Assay Time:3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of TNF-α in rat culture supernatants.
Wavelength:450 nm
Species reactivity:Rat
Use/Stability:Store all components at 4°C, except standard at -20°C.
Shipping:Shipped on Blue Ice
Contents:Microtiter plate, Conjugate, Antibody, Assay buffer 28, Wash buffer concentrate, Standard, TMB Substrate, Stop solution 2
Scientific Background:The TNF-α (rat), ELISA kit is a complete kit for the quantitative determination of rat TNF-α in culture supernatants. Please read the complete kit insert before performing this assay. Tumor necrosis factor-alpha (TNF-α), also known as cachectin, is a 17.5kDa, 157 amino acid member of the TNF superfamily of cytokines that is a potent lymphoid factor with effects on a wide range of target cells. Active TNF-α is produced as both soluble and membrane-anchored trimers by macrophages, NK cells, and T- and B-lymphocytes. TNF exerts proinflammatory signals via binding and inducing trimerization of TNF-receptor 1 (TNF-R1) expressed on most normal and transformed cells, or to TNF-receptor 2 (TNF-R2), expressed on endothelial and most immune cells. TNF signaling regulates hematopeiesis, differentiation, endothelial cell activation, apoptosis, lipid metabolism, tumor progression, and immune surveillance, and dysregulation of TNF or its receptors is implicated in numerous disease states including cancer, osteoporosis, autoimmune diseases, diabetes, and atherosclerosis.
Technical Info/Product Notes:Cited samples:
Cytokine ELISAs Cited Samples
UniProt ID:P16599
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Humulus japonicus extracts protect against lipopolysaccharide/d-galactosamine-induced acute liver injury in rats: J. Bae, et al.; J. Med. Food 21, 1009 (2018), Abstract;
Minocycline an antimicrobial agent attenuates the mitochondrial dependent cell death and stabilizes the expression of HIF-1α in spinal cord injury: G. Zhang, et al.; Microb. Pathog. (2018), Abstract;
Long-term feeding of red algae (Gelidium amansii) ameliorates glucose and lipid metabolism in a high fructose diet-impaired glucose tolerance rat model: H.C. Liu, et al.; J. Food Drug Anal. 25, 543 (2017), Application(s): Determination of tumor necrosis factor-α, rat plasma, Abstract;
Therapeutic role of ursolic acid on ameliorating hepatic steatosis and improving metabolic disorders in high-fat diet-induced non-alcoholic fatty liver disease rats: S. Li, et al.; PLoS One 9, e86724 (2014), Application(s): ELISA using rat serum, Abstract; Full Text

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