Alternative size available: ADI-901-070 (5x96 wells)
Sensitive measurement of LTC4/D4/E4, detecting as little as 26.6 pg/ml
High throughput format with results in 4 hours for up to 38 samples in duplicate
Fully quantitative results that surpass semi-quantitative Western blot analysis
The Cysteinyl Leukotriene EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 4 hours. Absorbance is read at 405 nm. Safe non-radioactive measurement of LTC4/D4/E4. Ready-to-use liquid color-coded reagents reduce errors.
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Product Details
Sensitivity:
26.6 pg/ml (range 78.1 - 2,500 pg/ml)
Assay Time:
4 hours
Applications:
ELISA, Colorimetric detection
Application Notes:
For the quantitative determination of Cysteinyl Leukotrienes (LTC4/D4/E4) in culture supernatants and urine from any species.
Wavelength:
405 nm
Species reactivity:
Species independent
Use/Stability:
Store all components at +4°C, except standard and conjugate at -20°C.
Leukotrienes are major products of 5-lipoxygenase metabolism of arachidonic acid. Leukotriene B4 (LTB4) stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation. The cysteinyl leukotrienes (LTC4, LTD4, and LTE4) are formed by addition of cysteine derivatives to LTA4. The conversion of LTA4 to LTC4 by the enzyme LTC4 synthase limits the rate of cysteinyl leukotriene formation. Cysteinyl leukotrienes are potent lipid mediators in inflammation, inducing smooth muscle contractions and increased capillary permeability.
Highly potent and selective 5-lipoxygenase inhibition by new, simple heteroaryl-substituted catechols for treatment of inflammation: V. Krauth, et al.; Biochem. Pharmacol. 208, 115385 (2023), Abstract;
Network analysis of large-scale ImmGen and Tabula Muris datasets highlights metabolic diversity of tissue mononuclear phagocytes: A. Gainullina, et al.; Cell Rep. 42, 112046 (2023), Abstract;
Cysteinyl leukotrienes and acetylcholine are biliary tuft cell cotransmitters: M. Keshavarz, et al.; Sci. Immunol. 7, eabf6734 (2022), Abstract;
Platelets control liver tumor growth through P2Y12-dependent CD40L release in NAFLD: C. Ma, et al.; Cancer Cell 40, 986 (2022), Abstract;
Cysteinyl leukotriene induces eosinophil extracellular trap formation via cysteinyl leukotriene 1 receptor in a murine model of asthma: A.A. da Cunha, et al.; Exp. Lung Res. 1080, 1902148 (2021), Abstract;
Attenuating effect of Peruvian cocoa populations on the acute asthmatic response in Brown Norway rats: M. Periz, et al.; Nutrients 12, 2301 (2020), Application(s): Analysis of rat BALF, Abstract; Full Text
Development and characterization of an allergic asthma rat model for interventional studies: M. Periz, et al.; Int. J. Mol. Sci. 21, 3841 (2020), Application(s): Analysis of rat BALF, Abstract; Full Text
Endogenous metabolites of vitamin E limit inflammation by targeting 5-lipoxygenase: H. Pein, et al.; Nat. Commun. 9, 3834 (2018), Application(s): Peritoneal exudates, Abstract; Full Text
Immunomodulatory and inhibitory effect of Immulina®, and Immunloges® in the Ig-E mediated activation of RBL-2H3 cells. A new role in allergic inflammatory: K. Appel, et al.; Plants 7, 13 (2018), Application(s): ELISA using culture supernatants, Abstract; Full Text
Urine concentrations changes of cysteinyl leukotrienes in non-obese children with obstructive sleep apnea undergoing adenotonsillectomy: G.K. Mousailidis, et al.; Int. J. Pediatr. Otorhinolaryngol. 114, 149 (2018), Application(s): Analysis of human urine samples, Abstract;
Advanced age in mares affects endometrial secretion of arachidonic acid metabolites during equine subclinical endometritis: M.J. Siemieniuch, et al.; Theriogenology 103, 191 (2017), Application(s): ELISA using culture supernatants, Abstract;
Identifying diagnostic endocrine markers and changes in endometrial gene expressions during pyometra in cats: E. Jursza-Piotrowska & M.J. Siemieniuch; Reprod. Biol. 16, 174 (2016), Application(s): Concentrations of leukotrienes C4 in plasma, Abstract;
Leukotriene B4 mediates macrophage influx and pulmonary hypertension in bleomycin-induced chronic neonatal lung injury: M.T. Ee, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 311, L292 (2016), Application(s): ELISA using rat lung extracts, Abstract;
Paediatric obstructive sleep apnoea syndrome (OSAS) is associated with tonsil colonisation by Streptococcus pyogenes: E. Viciani, et al.; Sci. Rep. 6, 20609 (2016), Application(s): ELISA using culture supernatants, Abstract; Full Text
The 5-lipoxygenase inhibitor RF-22c potently suppresses leukotriene biosynthesis in cellulo and blocks bronchoconstriction and inflammation in vivo: A.M. Schaible, et al.; Biochem. Pharmacol. 112, 60 (2016), Application(s): ELISA using culture supernatants, Abstract;
Vitex agnus-castus dry extract BNO 1095 (Agnucaston®) inhibits uterine hyper-contractions and inflammation in experimental models for primary dysmenorrhea: J. Rohrl, et al.; Int. J. Phytomed. Phytotherapy 2, 20 (2016), Application(s): Inhibition of purified 5-lipoxygenase (5-LO) and cellular leukotriene biosynthesis in isolated human monocytes,
Altered secretion of selected arachidonic acid metabolites during subclinical endometritis relative to estrous cycle stage and grade of fibrosis in mares: K. Gajos, et al.; Theriogenology 84, 457 (2015), Application(s): ELISA using mare sample, Abstract;
Cinnamaldehyde is the main mediator of cinnamon extract in mast cell inhibition: Y. Hagenlocher, et al.; Eur. J. Nutr. 54, 1297 (2015), Application(s): ELISA using culture supernatants, Abstract;
Elucidation of the molecular mechanism and the efficacy in vivo of a novel 1,4-benzoquinone that inhibits 5-lipoxygenase: A.M. Schaible, et al.; Br. J. Pharmacol. 171, 2399 (2014), Application(s): Cys-LT levels in culture supernatants of monocytes, Abstract; Full Text
Indirubin-3'-monoxime exerts a dual mode of inhibition towards leukotriene-mediated vascular smooth muscle cell migration: T. Blazevic, et al.; Cardiovasc. Res. 101, 522 (2014), Application(s): ELISA using culture supernatants, Abstract; Full Text
The novel benzimidazole derivative BRP-7 inhibits leukotriene biosynthesis in vitro and in vivo by targeting 5-lipoxygenase-activating protein (FLAP): C. Pergola, et al.; Br. J. Pharmacol. 171, 3051 (2014), Application(s): ELISA using culture supernatants, Abstract; Full Text
Effects of the rhizomes of Atractylodes japonica and atractylenolide I on allergic response and experimental atopic dermatitis: H. Lim, et al.; Arch. Pharm. Res. 35, 2007 (2012), Application(s): EIA using rat RBL-1 cells, Abstract;
