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12(S)-HETE ELISA kit

Sensitive ELISA kit enabling detection of 12(S)-HETE in culture supernatants and plasma from any species.
ADI-900-050 96 wells 483.00 USD
Do you need bulk/larger quantities?
Alternative size available: ADI-901-050 (5x96 wells)
  • Easy-to-use color-coded reagents and pre-coated plates reduce errors and save time in the lab
  • High throughput format, obtain results from up to 39 samples in duplicate in just 5 hours
  • Highly sensitive assay, measure as little as 146 pg/ml
  • Reliable and reproducible results day-after-day and lot-after-lot
The 12(S)-HETE EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 5 hours. Absorbance is read at 405 nm. Fast, accurate, and reliable results are obtained with ready-to-use liquid color-coded reagents that reduce error.
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Product Details

Alternative Name:12(S)-hydroperoxy tetraenoic eicosatetraenoic acid
Sensitivity:146 pg/ml (range 195 - 50,000 pg/ml)
Assay Time:5 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of 12(S)-HETE in culture supernatants and plasma from any species. Cited sample types include serum, renal interstitial fluid and tissue.
Wavelength:405 nm
Species reactivity:Species independent
Use/Stability:Store all components at +4°C, except standard and conjugate at -20°C.
Shipping:Shipped on Blue Ice
Contents:GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
Scientific Background:HETEs are byproducts generated by the metabolism of arachidonic acid by lipoxygenases. 12(S)-HETE is the stereospecific hydroxy product from the reduction of 12(S)-hydroperoxy tetraenoic eicosatetraenoic acid [12(S)-HpETE], which itself is a 12-lipoxygenase metabolite of arachidonic acid. 12(S)-HETE is chemotactic and chemokinetic for polymorphonuclear leukocytes and vascular smooth muscle cells. It also acts as a second messenger in angiotensin-II induced aldosterone production. 15-hydroxyeicosatetraenoic acid [15(S)-HETE] is the major hydroxy derivative of arachidonic acid when acted upon by 15-lipoxygenase. It is also the primary monohydroxy acid synthesized by the lipoxygenase activity of Cyclooxygenase-1 upon linoleic acid. 15(S)-HETE acts as a paracrine regulator of smooth muscle and lung neutrophil recruitment, and elevated levels of 15(S)-HETE are associated with asthma, rhinitis, chronic paranasal sinusitis and rheumatoid arthritis.
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Development of heart failure with preserved ejection fraction in type 2 diabetic mice is ameliorated by preserving vascular function: M. Otto, et al.; Life Sci. 284, 119925 (2021), Abstract;
12(S)-HETE Mediates Diabetes Induced Endothelial Dysfunction by Activating Intracellular Endothelial Cell TRPV1: M. Otto, et al.; J. Clin. Invest. 130, 4999 (2020), Abstract; Full Text
Arachidonate 12-Lipoxygenase and 12-Hydroxyeicosatetraenoic acid contribute to stromal aging-induced progression of pancreatic cancer: E.H. Sarsour, et al.; J. Biol. Chem. 295, 6946 (2020), Abstract; Full Text
A Mediterranean Diet Rich in Extra-Virgin Olive Oil Is Associated with a Reduced Prevalence of Nonalcoholic Fatty Liver Disease in Older Individuals at High Cardiovascular Risk: X. Pintó, et al.; J. Nutr. 149, 1920 (2019), Application(s): Human Urine, Abstract;
Ablation of TRPV1 Elevates Nocturnal Blood Pressure in Western Diet-fed Mice: B. Zhong, et al.; Curr. Hypertens. Rev. 15, 144 (2019), Application(s): Quantification of 12(S)-HETE in Urine, Abstract; Full Text
Mediatory roles of leukotriene B4 receptors in LPS-induced endotoxic shock: S.Y. Kwon, et al.; Sci. Rep. 9, 5936 (2019), Application(s): Mouse peritoneal lavage fluid and serum, Abstract; Full Text
5‐/12‐Lipoxygenase‐linked cascade contributes to the IL‐33‐induced synthesis of IL‐13 in mast cells, thus promoting asthma development: M. Ro, et al.; Allergy 73, 350 (2018), Application(s): Cell Culture Supernatant, Abstract;
Intravasation of SW620 colon cancer cell spheroids through the blood endothelial barrier is inhibited by clinical drugs and flavonoids in vitro: S. Holzner, et al.; Food Chem. Toxicol. 111, 114 (2018), Application(s): ELISA using culture supernatants, Abstract;
Mice lacking L-12/15-lipoxygenase show increased mortality during kindling despite demonstrating resistance to epileptogenesis: M.A. Kanzler, et al.; Epilepsia Open 3, 255 (2018), Application(s): Cell Culture Supernatant, Abstract; Full Text
Changes in platelet function with inflammation in patients undergoing vascular surgery: B. Olechowski, et al.; Platelets 30, 190 (2017), Application(s): Human Blood Serum, Abstract;
12(S)-HETE increases intracellular Ca2+ in lymph-endothelial cells disrupting their barrier function in vitro; stabilization by clinical drugs impairing calcium supply: C.H Nguyen, et al.; Cancer Lett. 380, 174 (2016), Application(s): Concentration of 12(S)-HETE in cellular supernatant, Abstract;
12-Lipoxygenase Inhibition on Microalbuminuria in Type-1 and Type-2 Diabetes Is Associated with Changes of Glomerular Angiotensin II Type 1 Receptor Related to Insulin Resistance: H.Z. Xu, et al.; Int. J. Mol. Sci. 17, E684 (2016), Application(s): Measurement of HETE levels in rats, Abstract; Full Text
Cancer cell-derived 12(S)-HETE signals via 12-HETE receptor, RHO, ROCK and MLC2 to induce lymph endothelial barrier breaching: C.H. Nguyen, et al.; Br. J. Cancer 115, 364 (2016), Application(s): 12(S)-HETE assay, rabbit, Abstract; Full Text
12/15-Lipoxygenase metabolites of arachidonic acid activate PPARγ: a possible neuroprotective effect in ischemic brain: L. Sun, et al.; J. Lipid. Res. 56, 502 (2015), Application(s): ELISA, Abstract; Full Text
Lobatin b inhibits NPM/ALK and NF-κB attenuating anaplastic-large-cell-lymphomagenesis and lymphendothelial tumour intravasation: I. Kiss, et al.; Cancer Lett. 356, 994 (2015), Application(s): ELISA using MCF7 cell culture supernatants, Abstract;
MyD88-BLT2-dependent cascade contributes to LPS-induced interleukin-6 production in mouse macrophage: A.J. Lee, et al.; Exp. Mol. Med. 47, e156 (2015), Application(s): ELISA using macrophage incubations, Abstract; Full Text
Pharmacologic blockade of 12/15-lipoxygenase ameliorates memory deficits, Aβ and tau neuropathology in the triple-transgenic mice: J. Chu, et al.; Mol. Psychiatry 20, 1329 (2015), Application(s): ELISA, Abstract;
Selective inhibition of 12-lipoxygenase protects islets and beta cells from inflammatory cytokine-mediated beta cell dysfunction: D.A. Taylor-Fishwick, et al.; Diabetologia 58, 549 (2015), Abstract;
Transient postnatal fluoxetine decreases brain concentrations of 20-HETE and 15-epi-LXA4, arachidonic acid metabolites in adult mice: Z.X. Yuan, et al.; Prostaglandins Leukot. Essent. Fatty Acids 101, 9 (2015), Application(s): ELISA using mouse brain samples, Abstract;
12-Lipoxygenase promotes obesity-induced oxidative stress in pancreatic islets: S.A. Tersey, et al.; Mol. Cell. Biol. 34, 3735 (2014), Application(s): ELISA using culture supernatants, Abstract;
The oxido-reductase enzyme glutathione peroxidase 4 (GPX4) governs Salmonella Typhimurium-induced neutrophil transepithelial migration: T.A. Agbor, et al.; Cell. Microbiol. 16, 1339 (2014), Application(s): ELISA using culture supernatants, Abstract; Full Text
In vitro inhibition of breast cancer spheroid-induced lymphendothelial defects resembling intravasation into the lymphatic vasculature by acetohexamide, isoxsuprine, nifedipin and proadifen: N. Kretschy, et al.; Br. J. Cancer 108, 570 (2013), Application(s): ELISA using MCF7 cell culture supernatants, Abstract; Full Text
Lipocalin-2 deficiency attenuates insulin resistance associated with aging and obesity: I.K. Law, et al.; Diabetes 59, 872 (2010), Application(s): EIA using mouse tissue homogenates, Abstract; Full Text
Role of 12/15-lipoxygenase in nitrosative stress and peripheral prediabetic and diabetic neuropathies: R. Stavniichuk, et al.; Free Radic. Biol. Med. 49, 1036 (2010), Application(s): EIA using mouse tissue homogenates, Abstract;
Effects of vitamin E on mitochondrial dysfunction and asthma features in an experimental allergic murine model: U. Mabalirajan, et al.; J. Appl. Physiol. 107, 1285 (2009), Application(s): EIA using mouse tissue homogenates, Abstract; Full Text
Foxp3 regulates megakaryopoiesis and platelet function: J.J. Bernard, et al.; Arterioscler. Thromb. Vasc. Biol. 29, 1874 (2009), Application(s): EIA using mouse serum and plasma, Abstract; Full Text
5-LOX, 12-LOX and 15-LOX in immature forms of human leukemic blasts: Y. Denizot, et al. ; Leuk. Res. 32, 1756 (2008), Application(s): EIA using human culture supernatants, Abstract;
Regulation of renal 12(S)-hydroxyeicosatetraenoic acid in diabetes by angiotensin AT1 and AT2 receptors: E.M. Abdel-Rahman, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 295, R1473 (2008), Application(s): EIA using mouse renal interstitial fluid, Abstract; Full Text

General Literature References

Oral green tea catechin metabolites are incorporated into human skin and protect against UV radiation-induced cutaneous inflammation in association with reduced production of pro-inflammatory eicosanoid 12-hydroxyeicosatetraenoic acid: L.E. Rhodes, et al.; Br. J. Nutr. 28, 1 (2013), Abstract;

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