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Urinary prostacyclin ELISA kit

ADI-900-025 96 wells 488.00 USD
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Alternative size available: ADI-901-025 (5x96 wells)
  • Quantitate PGI2 - measuring the stable breakdown products 6-keto-PGF and 2,3-dinor-6keto-PGF in one simple assay
  • Less stress - much easier than GC/MS with results from up to 39 samples in duplicate in under 3 hours
  • Specific - low cross-reactivity with related eicosanoids
The Urinary Prostacyclin EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. Quantitate PGI2 by measuring the stable metabolites in one simple assay.
Urinary Prostacyclin ELISA Kit
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Urinary Prostacyclin ELISA Kit

Product Details

Alternative Name:PGI2, Prostaglandin I2
Sensitivity:6.58 pg/ml (range 7.81 - 2,000 pg/ml)
Assay Time:<3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of urinary prostacyclin metabolites in culture supernatants and urine from any species.
Wavelength:405 nm
Species reactivity:Species independent
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Contents:DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
Scientific Background:Prostacyclin (PGI2) is involved in platelet aggregation, vasoconstriction, and reproductive functions. PGI2 has a half life of 60 minutes in plasma but only 2 to 3 minutes in buffer. The production of PGI2 is typically monitored by measurement of 6-keto-PGF and 2,3-dinor-6-keto-PGF. 6-keto-PGF is produced by the non-enzymatic hydration of PGI2, and is further metabolized in urine to the 2,3-dinor derivative. A number of pharmaceuticals alter and/or inhibit the synthesis of PGI2. Methods to measure PGI2 in urine typically involve HPLC, gas chromatography/mass spectrometry, or radioimmunoassay and enzyme immunoassay for 6-keto-PGF. The conversion of PGI2 into 6-keto-PGF and 2,3-dinor-6-keto-PGF is shown below.
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Hypothyroidism Affects Uterine Function via the Modulation of Prostaglandin Signaling: I. Kowalczyk-Zięba, et al.; Animals (Basel) 11, 2636 (2021), Application(s): Rat urine, Abstract; Full Text
Gelatin-based perfusable, endothelial carotid artery model for the study of atherosclerosis: R. Chen, et al.; Biomed. Eng. Online 18, 87 (2019), Abstract; Full Text
Identifying diagnostic endocrine markers and changes in endometrial gene expressions during pyometra in cats: E. Jursza-Piotrowska & M.J. Siemieniuch; Reprod. Biol. 16, 174 (2016), Application(s): Concentrations of prostacyclin (PGI2) in plasma, Abstract;
Red wine extract protects against oxidative-stress-induced endothelial senescence: I.P. Bolden, et al.; Clin. Sci. (Lond) 123, 499 (2012), Application(s): ELISA using culture supernatants, Abstract; Full Text

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