Big Endothelin-1 (human), ELISA kit



ADI-900-022	96 wells	944.00 USD
Do you need bulk/larger quantities?

Safe - non-radioactive determination of Big ET-1
Easy-to-use - less steps compared to Western blot
Reliable - consistent results lot-after-lot

The Big Endothelin-1 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results overnight + 1 hour.

Big Endothelin-1 (human), ELISA kit Kit graph

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Product Details

Alternative Name:	Big ET-1

Sensitivity:	0.30 pg/ml (range 0.78 - 100 pg/ml)

Assay Time:	Overnight + 1 hour

Applications:	ELISA, Colorimetric detection

Application Notes:	For the quantitative determination of human Big ET-1 in culture supernatants, serum, and EDTA plasma. Cited species and sample type include rabbit and lung lavage fluid.

Wavelength:	450 nm

Species reactivity:	Human

Shipping:	Blue Ice Not Frozen

Long Term Storage:	+4°C

Contents:	Microtiter plate, Labeled antibody concentrate, Antibody diluent, Assay buffer, Wash buffer concentrate, Standard, TMB Substrate, Stop solution

Scientific Background:	Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ET_A and ET_B. Activation of ET_A increases vasoconstriction and sodium retention, thereby increasing blood pressure. Activation of ET_B receptors triggers the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells and increases urine production and elimination, thereby lowering blood pressure.

UniProt ID:	P05305

Regulatory Status:	RUO - Research Use Only

Compatibility:	This product is compatible with the Absorbance 96 Plate Reader.

Product Literature References

Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts: L. Mahemuti, et al.; J. Environ. Sci. (China) 48, 11 (2016), Application(s): Collected and analyzed supernatants, Abstract;

Elevated immunoreactive endothelin-1 levels in newborn rabbit lungs after meconium aspiration: A. Zagariya, et al. ; Pediatr. Crit. Care Med. 3, 297 (2002), Application(s): EIA using rabbit lung lavage fluid, Abstract;

Endothelin-1 Is Induced by Cytokines in Human Vascular Smooth Muscle Cells: Evidence for Intracellular Endothelin-Converting Enzyme: M. Woods, et al. ; Mol. Pharmacol. 55, 902 (1999), Application(s): EIA using human culture supernatants, Abstract;