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Big Endothelin-1 (human), ELISA kit

ADI-900-022 96 wells 972.00 USD
Do you need bulk/larger quantities?
  • Safe - non-radioactive determination of Big ET-1
  • Easy-to-use - less steps compared to Western blot
  • Reliable - consistent results lot-after-lot
The Big Endothelin-1 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results overnight + 1 hour.
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Product Details

Alternative Name:Big ET-1
Sensitivity:0.30 pg/ml (range 0.78 - 100 pg/ml)
Assay Time:Overnight + 1 hour
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of human Big ET-1 in culture supernatants, serum, and EDTA plasma. Cited species and sample type include rabbit and lung lavage fluid.
Wavelength:450 nm
Species reactivity:Human
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Contents:Microtiter plate, Labeled antibody concentrate, Antibody diluent, Assay buffer, Wash buffer concentrate, Standard, TMB Substrate, Stop solution
Scientific Background:Endothelins (ET) are isopeptides produced by vascular endothelium that have potent vasoconstrictive activity. The peptides are encoded by three separate genes and processed to yield 39-residue "Big ET" molecules which are further processed to the 21 amino acid sequences designated ET-1, ET-2 and ET-3. ET action is mediated via binding to two receptors, ETA and ETB. Activation of ETA increases vasoconstriction and sodium retention, thereby increasing blood pressure. Activation of ETB receptors triggers the release of vasorelaxive factors such as nitric oxide (NO) and prostanoids from endothelial cells and increases urine production and elimination, thereby lowering blood pressure.
UniProt ID:P05305
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Bisphenol A exposure alters release of immune and developmental modulators and expression of estrogen receptors in human fetal lung fibroblasts: L. Mahemuti, et al.; J. Environ. Sci. (China) 48, 11 (2016), Application(s): Collected and analyzed supernatants, Abstract;
Elevated immunoreactive endothelin-1 levels in newborn rabbit lungs after meconium aspiration: A. Zagariya, et al. ; Pediatr. Crit. Care Med. 3, 297 (2002), Application(s): EIA using rabbit lung lavage fluid, Abstract;
Endothelin-1 Is Induced by Cytokines in Human Vascular Smooth Muscle Cells: Evidence for Intracellular Endothelin-Converting Enzyme: M. Woods, et al. ; Mol. Pharmacol. 55, 902 (1999), Application(s): EIA using human culture supernatants, Abstract;

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