- Flexible - broad dynamic range suitable for many normal and pathological samples
- Sensitive - measure < 26 pg/ml mouse leptin
- Fast - fully quantitative results in just 3 hours from up to 39 samples in duplicate
- Pre-coated plates and ready-to-use reagents save you time
The Leptin (mouse), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. The broad dynamic range is suitable for many normal and pathological samples with sensitive quantification of < 26 pg/mL mouse leptin.
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Product Details
| Alternative Name: | OB gene product |
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| Sensitivity: | 25.4 pg/ml (range 50 - 3,200 pg/ml) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of mouse leptin in culture supernatants, plasma, and serum. Cited sample type includes tissue. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Mouse
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | Microtiter plate, Standard, Antibody, Conjugate, Assay buffer 28, TMB Substrate, Stop solution 2, Wash buffer concentrate |
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| Scientific Background: | The adipokine leptin is a hormone secreted predominantly by adipose tissue that signals through leptin receptors in the hypothalamus to decrease appetite and increase energy expenditure. Binding of leptin to the long-form of the leptin receptor in the hypothalamus reduces neuropeptide Y (NPY) and agouti-related protein (AgRP) activity, while stimulating anorexigenic pro-opiomelanocortin (POMC) neuron activity to reduce appetite. In peripheral tissues, leptin antagonizes insulin signaling, increases fatty acid oxidation, decreases insulin production in pancreatic β cells, and promotes fertility. Its expression in adipocytes can be regulated in a paracrine fashion by other adipokines such as IL-6 (stimulation) and TNF-α (inhibition).
Leptin deficient mice display severe insulin resistance, obesity, and decreased fertility, all of which are reversible by administration of exogenous leptin. In contrast, elevated circulating levels of leptin are associated with increased obesity, indicative of an acclimated state of leptin resistance which is not well understood. Evidence also links leptin to cardiovascular disease, as it mediates numerous pro-inflammatory and pro-atherogenic effects such as increased endothelin-1 (ET-1) expression, accumulation of reactive oxygen species (ROS), secretion of monocyte chemoattractant protein-1 (MCP-1), and increased sympathetic vascular tone and blood pressure. |
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| Technical Info/Product Notes: | Cited samples:
Cytokine ELISAs Cited Samples |
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| UniProt ID: | P41160 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
High salt diet does not impact the development of acute myeloid leukemia in mice: M. Janakiraman, et al.; Cancer Immunol. Immunother.
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Abstract;
eNAMPT actions through nucleus accumbens NAD+/SIRT1 link increased adiposity with sociability deficits programmed by peripuberty stress: L. Morató, et al.; Sci. Adv.
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Abstract;
Transcription factor CREB3 is a potent regulator of high-fat diet-induced obesity and energy metabolism: B.S. Smith, et al.; Int. J. Obes.
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Abstract;
The effects of mirabegron on obesity-induced inflammation and insulin resistance are associated with brown adipose tissue activation but not beiging in the subcutaneous white adipose tissue: C. Peres Valgas da Silva, et al.; Clin. Exp. Pharmacol. Physiol.
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Abstract;
Anti-Obesity Effects of Collagen Peptide Derived from Skate (Raja kenojei) Skin Through Regulation of Lipid Metabolism: M. Woo, et al.; Mar. Drugs
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Abstract;
Full Text
Anti-obesity effects of pectinase and cellulase enzyme‑treated Ecklonia cava extract in high‑fat diet‑fed C57BL/6N mice: I.H. Kim, et al.; Int. J. Mol. Med.
41, 924 (2018),
Application(s): ELISA using mouse serum,
Abstract;
Full Text
Euphorbia supina extract results in inhibition of high‑fat‑diet‑induced obesity in mice: S. Nepali, et al.; Int. J. Mol. Med.
41, 2952 (2018),
Abstract;
Hypolipidemic and antidiabetic effects of functional rice cookies in high-fat diet-fed ICR mice and db/db mice: S.H. Hong, et al.; J. Med. Food
21, 535 (2018),
Abstract;
Effects of Erythrocyte Membrane Polyunsaturated Fatty Acids in Overweight, Obese, and Morbidly Obese Korean Women: H.N. Choi, et al.; J. Cancer Prev.
22, 182 (2017),
Application(s): Human serum,
Abstract;
Full Text
Metabolic, epigenetic, and transgenerational effects of gut bacterial choline consumption: K.A. Romano, et al.; Cell Host Microbe
22, 279 (2017),
Application(s): ELISA using mouse serum,
Abstract;
Full Text
The metabolic ER stress sensor IRE1α suppresses alternative activation of macrophages and impairs energy expenditure in obesity: B. Shan, et al.; Nat. Immunol.
18, 519 (2017),
Abstract;
Anti-obesity effects of boiled tuna extract in mice with obesity induced by a high-fat diet: Y. Kim, et al.; Int. J. Mol. Med.
38, 1281 (2016),
Application(s): Mouse serum,
Abstract;
High fat diet augments amphetamine sensitization in mice: Role of feeding pattern, obesity, and dopamine terminal changes: S. C. Fordahl, et al. ; Neuropharmacology
109, 170 (2016),
Application(s): Leptin mouse plasma hormones were measured,
Abstract;
Increased apoptosis and browning of TAK1-deficient adipocytes protects against obesity: A. Sassmann-Schweda, et al.; JCI Insight
1, e81175 (2016),
Application(s): Measured mouse plasma levels,
Abstract;
Full Text
Nucleus accumbens cocaine-amphetamine regulated transcript mediates food intake during novelty conflict: P.R. Burghardt, et al.; Physiol. Behav.
158, 76 (2016),
Application(s): Levels of leptin were measured in blood samples,
Abstract;
The NLRP3 inflammasome is dispensable for ER stress-induced pancreatic β-cell damage in Akita mice: J. Wang, et al.; Biochem. Biophys. Res. Commun.
466, 300 (2015),
Application(s): ELISA kit measuring plasma leptin levels in mice,
Abstract;
Effects of isolated GH deficiency on adipose tissue, feeding and adipokines in mice: L. Recinella, et al.; Growth Horm. IGF Res.
23, 237 (2013),
Application(s): ELISA using mouse serum,
Abstract;
Adipogenic human adenovirus-36 reduces leptin expression and secretion and increases glucose uptake by fat cells: S.D. Vangipuram, et al.; Int. J. Obes. (Lond.)
31, 87 (2007),
Application(s): EIA using cell culture supernatant,
Abstract;
Full Text
Leptin improves pulmonary bacterial clearance and survival in ob/ob mice during pneumococcal pneumonia: A. Hsu, et al.; Clin. Exp. Immunol.
150, 332 (2007),
Application(s): EIA using mouse tissue homogenate,
Abstract;
Full Text
Folliculogenesis is impaired and granulosa cell apoptosis is increased in leptin-deficient mice: M.L. Hamm, et al.; Biol. Reprod.
71, 66 (2004),
Application(s): EIA using mouse serum,
Abstract;
Full Text
Does leptin mediate the effect of photoperiod on immune function in mice?: G.K. Bhat, et al.; Biol. Reprod.
69, 30 (2003),
Application(s): EIA using mouse serum,
Abstract;
Full Text
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