Alternative size available: ADI-901-018A (5x96 wells)
Highly sensitive assay, measuring as little as 5.3 pg/ml of Substance P
Widely cited in numerous peer reviewed publications
High throughput fomat, assay up to 38 samples in duplicate per 96 well kit
Fully quantitative results that surpass semi-quantitative Western blot analysis
The Substance P EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. This kit utilized low sample volume and provides the ability to assay up to 38 samples in duplicate per 96 well kit.
Product Details
Sensitivity:
5.3 pg/ml (range 9.76 - 10,000 pg/ml)
Assay Time:
3 hours
Applications:
ELISA, Colorimetric detection
Application Notes:
For the quantitative determination of Substance P in culture supernatants, plasma, serum, saliva, and urine from any species. Cited sample type includes tissue.
Wavelength:
405 nm
Species reactivity:
Species independent
Use/Stability:
Store components at temperatures indicated on the component labeling. The Antibody, Conjugate and Standard are stored at -20°C and the remaining components are stored at 4°C. For convenience upon receipt, the unopened kit containing all components can be stored at -20°C and are stable at that temperature until the kit’s expiration date. After first thaw, store components at temperature indicated on the labeling as indicated above. Freeze-thaw cycles should be limited for optimal kit performance. Please see Reagent Preparation section in the product manual for more detailed component handling information.
Substance P is an undecapeptide hormone (RPKPQQFFGLM) that mediates a number of biological activities including nausea, anxiety, pain, and vasodilation. It is a member of the tachykinin family of neuropeptides, and is widely distributed in the peripheral and central nervous systems of vertebrates.
Evaluation of Substance P as a New Stress Parameter in Horses in a Stress Model Involving Four Different Stress Levels: D. Scholler, et al.; Animals 13, 1142 (2023), Abstract;
Functional validation of co-culture model of human keratinocytes and neuronal cell line for sensitive skin by using transient receptor potential channel vanilloid subfamily member 1 antagonist: S.M. Shin, et al.; Skin Res. Technol. 29, e13275 (2023), Abstract;
A Potential Role for Substance P in West Nile Virus Neuropathogenesis: S.E. Ronca, et al.; Viruses 14, 1961 (2022), Abstract;
Elevated serum substance P during simian varicella virus infection in rhesus macaques: implications for chronic inflammation and adverse cerebrovascular events: A. Bubak, et al.; J. Neurovirol. 26, 945 (2020), Abstract;
Role of the afferent renal nerves in sodium homeostasis and blood pressure regulation in rats: A.A. Frame, et al.; Exp. Physiol. 104, 1306 (2019), Application(s): ELISA using culture supernatants, Abstract;
Sensory neuropeptides and their receptors participate in mechano-regulation of murine macrophages: D. Muschter, et al.; Int. J. Mol. Sci. 20, 503 (2019), Application(s): ELISA using culture supernatants, Abstract; Full Text
Aggressive attempted escape behavior during head-lock restraint reduced reproductive performances in Holstein heifers: V.R. Kasimanickam, et al.; Theriogenology 121, 147 (2018), Application(s): ELISA using bovine serum, Abstract;
Effects of castration and a lidocaine-plus-flunixin treatment on growth and indicators of pain, inflammation, and liver function in Korean cattle bull calves: S.J. Park, et al.; Livestock Sci. 216, 61 (2018), Application(s): Bovine Plasma,
Predictors of beef calf temperament at weaning and its impact on temperament at breeding and reproductive performance: V.R. Kasimanickam, et al.; Reprod. Domest. Anim. 53, 484 (2018), Application(s): ELISA using bovine serum, Abstract;
Saliva as a medium to detect and measure biomarkers related to pain: H. Jasim, et al.; Sci. Rep. 8, 3220 (2018), Application(s): ELISA using human plasma and saliva, Abstract; Full Text
Effects on breathing of agonists to μ-opioid or GABAA receptors dialyzed into the Ventral Respiratory Column of awake and sleeping goats: T.M. Langer, et al.; Respir. Physiol. Neurobiol. 239, 10 (2017), Abstract;
Flunixin meglumine improves pregnancy rate in embryo recipient beef cows with an excitable temperament: R.K. Kasimanickam, et al.; Theriogenology 107, 70 (2017), Abstract; Full Text
Acid-Sensing Ion Channel 1a Contributes to Airway Hyperreactivity in Mice: L.R. Reznikov, et al.; PLoS One 11, e0166089 (2016), Abstract; Full Text
Efficacy of oral meloxicam suspension for prevention of pain and inflammation following band and surgical castration in calves: M. Olson, et al.; BMC Vet. Res. 12, 102 (2016), Application(s): Measurment of substance P in blood samples, Abstract; Full Text
Evolution of Chronic Pain in Sickle Cell Disease: M. Pope, et al.; Blood 128, 1297 (2016), Full Text
Substance P Receptor Signaling Mediates Doxorubicin-Induced Cardiomyocyte Apoptosis and Triple-Negative Breast Cancer Chemoresistance: P. Robinson, et al.; Biomed. Res. Int. 2016, 1959270 (2016), Abstract; Full Text
Substance P Receptor Signaling Mediates Doxorubicin-Induced Cardiomyocyte Apoptosis Triple-Negative Breast Cancer Chemoresistance: P. Robinson, et al.; Biomed. Res. Int. 2016, Article ID 1959270 (2016), Application(s): Quantitation of SP Protein in cell lysates, Abstract; Full Text
The role of substance P in the maintenance of colonic hypermotility induced by repeated stress in rats: P. Lu, et al.; Neuropeptides 56, 75 (2016), Application(s): Determine levels of substance P in serum, Abstract;
Blockade of neurokinin-1 receptors in the ventral respiratory column does not affect breathing but alters neurochemical release: C. Muere, et al.; J. Appl. Physiol. 118, 732 (2015), Application(s): ELISA, Abstract;
Characteristics and Potential Biomarkers for Chronic Pain in Patients with Sickle Cell Disease: N. Kuei, et al.; Blood 126, 986 (2015), Application(s): Human Substance P levels were measured, Full Text
Neuropeptide release augments serum albumin loss and reduces ultrafiltration in peritoneal dialysis: N. Cavallini, et al.; Perit. Dial. Int. 32, 168 (2012), Abstract; Full Text
Effect of anti-NGF antibodies in a rat tibia fracture model of complex regional pain syndrome type I : I. Sabsovich, et al. ; Pain 138, 47 (2008), Application(s): EIA using tissue, Abstract;
Endogenous ATP potentiates only vasopressin secretion from neurohypophysial terminals: J. Lemos, et al. ; J. Cell. Physiol. 217, 155 (2008), Application(s): EIA using rat culture supernatants, Abstract;
Tachykinin neurokinin 3 receptor signaling in cholecystokinin-elicited release of oxytocin and vasopressin: F. Flynn, et al. ; Am. J. Physiol. Regul. Integr. Comp. Physiol. 294, R1760 (2008), Application(s): EIA using rat plasma, Abstract; Full Text
Loss of Vav2 proto-oncogene causes tachycardia and cardiovascular disease in mice: X. Bustelo, et al. ; Mol. Biol. Cell 18, 943 (2007), Application(s): EIA using mouse plasma, Abstract;
Tachykinin NK3 receptor contribution to systemic release of vasopressin and oxytocin in response to osmotic and hypotensive challenge: G.E. Haley, et al. ; Am. J. Physiol. Regul. Integr. Comp. Physiol. 293, R931 (2007), Application(s): EIA using rat plasma, Abstract; Full Text
Urea transporter UT-A1 and aquaporin-2 proteins decrease in response to angiotensin II or norepinephrine-induced acute hypertension: J.D. Klein, et al. ; Ren. Physiol. 291, F952 (2006), Application(s): EIA using rat plasma, Abstract;
