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No edge effect - all incubations at room temperature eliminate risk of evaporation at 37°C
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Broad dynamic range - easily quantify high and low levels of leptin
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Convenient- store all reagents at 4°C
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Fast - fully quantitative results in just 3 hours from up to 39 samples in duplicate
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Pre-coated plates and ready-to-use reagents save you time
The Leptin (rat), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours.
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Product Details
| Alternative Name: | OB gene product |
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| Sensitivity: | 67.2 pg/ml (range 100 - 6,400 pg/ml) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of rat leptin in culture supernatants, plasma, and serum. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Rat
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | Microtiter plate, Standard, Antibody, Conjugate, Assay buffer 28, TMB Substrate, Stop solution 2, Wash buffer concentrate |
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| Scientific Background: | The adipokine leptin is a hormone secreted predominantly by adipose tissue that signals through leptin receptors in the hypothalamus to decrease appetite and increase energy expenditure. Binding of leptin to the long-form of the leptin receptor in the hypothalamus reduces neuropeptide Y (NPY) and agouti-related protein (AgRP) activity, while stimulating anorexigenic pro-opiomelanocortin (POMC) neuron activity to reduce appetite. In peripheral tissues, leptin antagonizes insulin signaling, increases fatty acid oxidation, decreases insulin production in pancreatic beta cells, and promotes fertility. Its expression in adipocytes can be regulated in a paracrine fashion by other adipokines such as IL-6 (stimulation) and TNF-α (inhibition). Leptin deficient mice display severe insulin resistance, obesity, and decreased fertility, all of which are reversible by administration of exogenous leptin. In contrast, elevated circulating levels of leptin are associated with increased obesity, indicative of an acclimated state of leptin resistance which is not well understood. |
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| Technical Info/Product Notes: | Cited samples:
Cytokine ELISAs Cited Samples |
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| UniProt ID: | P50596 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Altered motor, anxiety-related and attentional task performance at baseline associate with multiple gene copies of the vesicular acetylcholine transporter and related protein overexpression in ChAT::Cre+ rats: C.P. Mantanona, et al.; Brain Struct. Funct.
224, 3095 (2019),
Abstract;
Full Text
Long-term feeding of red algae (Gelidium amansii) ameliorates glucose and lipid metabolism in a high fructose diet-impaired glucose tolerance rat model: H.C. Liu, et al.; J. Food Drug Anal.
25, 543 (2017),
Application(s): Determination of leptin, rat plasma,
Abstract;
Melatonin supplementation plus exercise behavior ameliorate insulin resistance, hypertension and fatigue in a rat model of type 2 diabetes mellitus: M.M. Rahman, et al.; Biomed. Pharmacother.
92, 606 (2017),
Abstract;
Effect of Intermittent Hypoxia on Arcuate Nucleus in the Leptin-Deficient Rat: J. Ciriello, et al.; Neurosci. Lett.
626, 112 (2016),
Application(s): Measured leptin levels in rat aqueous plasma phase,
Abstract;
Neonatal treatment with scopolamine butylbromide prevents metabolic dysfunction in male rats: A. Malta, et al.; Sci. Rep.
6, 30745 (2016),
Application(s): Leptin level quantification,
Abstract;
Full Text
Impact of prenatal exposure to obesogenic environment on later-life brown and white adipocytes and liver outcomes: L. Bezpalko, et al.; Proc. Shevchenko Sci. Soc. Medicine
41, 33 (2015),
Application(s): ELISA using rat serum,
Full Text
Lactobionic acid reduces body weight gain in diet-induced obese rats by targeted inhibition of galectin-1: R. Mukherjee, et al.; Biochem. Biophys. Res. Commun.
463, 1311 (2015),
Application(s): ELISA using rat plasma,
Abstract;
Red algae (Gelidium amansii) reduces adiposity via activation of lipolysis in rats with diabetes induced by streptozotocin-nicotinamide: T. Yang, et al.; J. Food Drug Anal.
23, 758 (2015),
Application(s): ELISA using rat plasma,
Abstract;
Acylated Ghrelin Limits Fat Accumulation and Improves Redox State and Inflammation Markers in the Liver of High-Fat-Fed Rats: R. Barazzoni, et al.; Obesity (Silver Spring)
22, 170 (2014),
Application(s): EIA using rat plasma,
Abstract;
Therapeutic role of ursolic acid on ameliorating hepatic steatosis and improving metabolic disorders in high-fat diet-induced non-alcoholic fatty liver disease rats: S. Li, et al.; PLoS One
9, e86724 (2014),
Application(s): ELISA using rat serum,
Abstract;
Full Text
Effects of intermittent hypoxia on leptin signalling in the carotid body: S.A. Messenger, et al.; Neuroscience
232, 216 (2013),
Application(s): ELISA using rat plasma,
Abstract;
Gender dimorphism in regulation of plasma proteins in streptozotocin-induced diabetic rats: J.W. Choi, et al.; Proteomics
13, 2482 (2013),
Application(s): Rat plasma,
Abstract;
Gender-dimorphic regulation of skeletal muscle proteins in streptozotocin-induced diabetic rats: M. Choi, et al.; Cell Physiol. Biochem.
31, 408 (2013),
Application(s): EIA using rat plasma,
Abstract;
Full Text
Imbalance of leptin pathway and hypothalamus synaptic plasticity markers are associated with stress-induced depression in rats: J.F. Ge, et al.; Behav. Brain Res.
249, 38 (2013),
Application(s): EIA using rat serum,
Abstract;
Overweight, hypertension and renal dysfunction in adulthood of neonatally overfed rats: H.E. Yim, et al.; J. Nutr. Biochem.
24, 1324 (2013),
Application(s): EIA using rat plasma,
Abstract;
Poor pubertal protein nutrition disturbs glucose-induced insulin secretion process in pancreatic islets and programs rats in adulthood to increase fat accumulation: J.C. de Oliveira, et al.; J. Endocrinol.
216, 195 (2013),
Application(s): EIA using rat plasma,
Abstract;
Full Text
Anticontractile Effect of Perivascular Adipose Tissue and Leptin are Reduced in Hypertension: B. Gálvez-Prieto, et al.; Front. Pharmacol.
3, 103 (2012),
Application(s): EIA using rat plasma,
Abstract;
Full Text
Intermittent hypoxia and systemic leptin administration induces pSTAT3 and Fos/Fra-1 in the carotid body: S.A. Messenger, et al.; Brain Res.
1446, 56 (2012),
Application(s): ELISA using rat plasma,
Abstract;
Role of NF-κB and p38 MAPK activation in mediating angiotensin II and endothelin-1-induced stimulation in leptin production and cardiomyocyte hypertrophy: V. Rajapurohitam, et al.; Mol. Cell. Biochem.
366, 287 (2012),
Abstract;
Leptin-mediated hypothalamic pathway of cholecystokinin (CCK-8) to regulate body weight in free-feeding rats: B. Merino, et al.; Endocrinology
149, 1994 (2008),
Application(s): EIA using rat plasma,
Abstract;
Full Text
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