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6-keto-PGF ELISA kit

Highly sensitive ELISA, detecting as low as 1.40 pg/ml in just 3 hours.
 
ADI-900-004 96 wells 368.00 USD
Do you need bulk/larger quantities?
 
Alternative size available: ADI-901-004 (5x96 wells)
  • Fast results from up to 38 samples in duplicate in just 3 hours
  • Highly specific with low cross-reactivity with structurally related molecules
  • Reliable and fully validated in-house with a variety of matrices
  • Widely published in peer-reviewed journals
The 6-keto-PGF EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. This is a highly specific kit with low cross-reactivity to structurally related molecules. The 6-keto-PGF EIA kit has been fully validated in-house in a wide variety of sample matrices.
6-keto-PGF1&alpha; ELISA kit Kit graph
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6-keto-PGF1&alpha; ELISA kit Kit graph

Product Specification

Alternative Name:6-keto Prostaglandin F
 
Sensitivity:1.40 pg/ml (range 3.2 - 50,000 pg/ml)
 
Assay Time:<3 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of 6-keto-PGF in culture supernatants, serum, saliva, and urine from any species.
 
Species reactivity:Species independent
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Contents:DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
 
Scientific Background:6-keto-PGF is a stable hydrolysis product of prostacyclin (PGI2). Due to the short half-life of PGI2 in buffer (2-3 minutes) the production of prostacyclin is typically monitored by measurement of the 6-keto-PGF metabolite and its derivative in urine, 2,3-dinor-6-keto-PGF. PGI2 has autocoid functions that include platelet aggregation, inhibition of vasoconstrictor responses to pressor hormones, platelet anti- and disaggregatory properties, and reproductive functions. The effects are induced by receptor-mediated elevation of cAMP. Prostacyclin has clinical applications in atherosclerosis and protection from myocardial ischemia.
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Aspirin blocks formation of metastatic intravascular niches by inhibiting platelet-derived COX-1/thromboxane A2: S. Lucotti, et al.; J. Clin. Invest. 129, 1845 (2019), Application(s): Mouse blood plasma, Abstract; Full Text
Evidence for increased content of PGF2α, PGE2 and 6-keto-PGF1α in endometrial tissue cultures from heavy draft mares in anestrus with endometritis: M.J. Siemieniuch, et al.; J. Equine Vet. Sci. 77, 107 (2019), Application(s): ELISA using culture supernatants, Abstract;
Alterations in NO- and PGI2- dependent function in aorta in the orthotopic murine model of metastatic 4T1 breast cancer: relationship with pulmonary endothelial dysfunction and systemic inflammation: E. Buczek, et al.; BMC Cancer 18, 582 (2018), Application(s): Culture medium from isolated mouse aortic rings, Abstract; Full Text
Inhibitory Effects of an Orally Active Thromboxane A2 Receptor Antagonist, nstpbp5185, on Atherosclerosis in ApoE-Deficient Mice: S.W. Huang, et al.; Thromb. Haemost. 118, 401 (2018), Abstract;
Advanced age in mares affects endometrial secretion of arachidonic acid metabolites during equine subclinical endometritis: M.J. Siemieniuch, et al.; Theriogenology 103, 191 (2017), Application(s): ELISA using culture supernatants, Abstract;
Colon cancer-derived myofibroblasts increase endothelial cell migration by glucocorticoid-sensitive secretion of a pro-migratory factor: Z. Drebert, et al.; Vascul. Pharmacol. 89, 19 (2017), Application(s): Determine the concentrations of prostanoids in conditioned medium from myofibroblasts and HUVECs, and in HUVEC lysates, Abstract;
Effect of Regular Aerobic Activity in Young Healthy Athletes on Profile of Endothelial Function and Platelet Activity: K. Podgórska, et al.; BioMed Res. Int. 2017, 8715909 (2017), Application(s): Human plasma, Abstract; Full Text
Protective Effects of Total Flavones of Elaeagnus rhamnoides (L.) A. Nelson against Vascular Endothelial Injury in Blood Stasis Model Rats: Z. Wei, et al.; Evid. Based Complement. Alternat. Med. 2017, 8142562 (2017), Abstract; Full Text
Altered secretion of selected arachidonic acid metabolites during subclinical endometritis relative to estrous cycle stage and grade of fibrosis in mares: K. Gajos, et al.; Theriogenology 84, 457 (2015), Application(s): ELISA using mare sample, Abstract;
Effect of Cyclooxygenase(COX)-1 and COX-2 inhibition on furosemide-induced renal responses and isoform immunolocalization in the healthy cat kidney: L. Pelligand, et al.; BMC Vet. Res. 11, 296 (2015), Abstract; Full Text
Endothelial dysfunction in young healthy men is associated with aspirin resistance: A. Doroszko, et al.; Vascul. Pharmacol. 67-69, 30 (2015), Application(s): ELISA using human blood, Abstract;
Urea-induced ROS cause endothelial dysfunction in chronic renal failure: M. D'Apolito, et al.; Atherosclerosis 239, 393 (2015), Application(s): ELISA, Abstract;
Carnosol and carnosic acids from Salvia officinalis inhibit microsomal prostaglandin E2 synthase-1: J. Bauer, et al.; J. Pharmacol. Exp. Ther. 342, 169 (2012), Application(s): ELISA using human cell culture supernatants, Abstract;
Arzanol, a prenylated heterodimeric phloroglucinyl pyrone, inhibits eicosanoid biosynthesis and exhibits anti-inflammatory efficacy in vivo: J. Bauer, et al.; Biochem. Pharmacol. 81, 259 (2011), Application(s): ELISA using human cell culture supernatants, Abstract;

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