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Fast results from up to 38 samples in duplicate in just 3 hours
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Highly specific with low cross-reactivity with structurally related molecules
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Reliable and fully validated in-house with a variety of matrices
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Widely published in peer-reviewed journals
The 6-keto-PGF1α EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. This is a highly specific kit with low cross-reactivity to structurally related molecules. The 6-keto-PGF1α EIA kit has been fully validated in-house in a wide variety of sample matrices.
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Product Details
| Alternative Name: | 6-keto Prostaglandin F1α |
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| Sensitivity: | 1.40 pg/ml (range 3.2 - 50,000 pg/ml) |
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| Assay Time: | <3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of 6-keto-PGF1α in culture supernatants, serum, saliva, and urine from any species. |
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| Wavelength: | 405 nm |
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| Species reactivity: | Species independent
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
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| Scientific Background: | 6-keto-PGF1α is a stable hydrolysis product of prostacyclin (PGI2). Due to the short half-life of PGI2 in buffer (2-3 minutes) the production of prostacyclin is typically monitored by measurement of the 6-keto-PGF1α metabolite and its derivative in urine, 2,3-dinor-6-keto-PGF1α. PGI2 has autocoid functions that include platelet aggregation, inhibition of vasoconstrictor responses to pressor hormones, platelet anti- and disaggregatory properties, and reproductive functions. The effects are induced by receptor-mediated elevation of cAMP. Prostacyclin has clinical applications in atherosclerosis and protection from myocardial ischemia. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Contribution of cyclooxygenase-1-dependent prostacyclin synthesis to bradykinin-induced dermal extravasation: M. Krybus, et al.; Biomed. Pharmacother.
148, 112786 (2022),
Abstract;
Differential effects of cyclo-oxygenase 1 and 2 inhibition on angiogenesis inhibitor-induced hypertension and kidney damage: K.M. Mirabito Colafella, et al.; Clin. Sci. (Lond.)
136, 675 (2022),
Abstract;
Estrogen modulates metabolic risk profile after resistance training in early postmenopausal women: a randomized controlled trial: T.V. Dam, et al.; Menopause
28, 1214 (2021),
Abstract;
Aspirin blocks formation of metastatic intravascular niches by inhibiting platelet-derived COX-1/thromboxane A2: S. Lucotti, et al.; J. Clin. Invest.
129, 1845 (2019),
Application(s): Mouse blood plasma,
Abstract;
Full Text
Evidence for increased content of PGF2α, PGE2 and 6-keto-PGF1α in endometrial tissue cultures from heavy draft mares in anestrus with endometritis: M.J. Siemieniuch, et al.; J. Equine Vet. Sci.
77, 107 (2019),
Application(s): ELISA using culture supernatants,
Abstract;
Alterations in NO- and PGI2- dependent function in aorta in the orthotopic murine model of metastatic 4T1 breast cancer: relationship with pulmonary endothelial dysfunction and systemic inflammation: E. Buczek, et al.; BMC Cancer
18, 582 (2018),
Application(s): Culture medium from isolated mouse aortic rings,
Abstract;
Full Text
Inhibitory Effects of an Orally Active Thromboxane A2 Receptor Antagonist, nstpbp5185, on Atherosclerosis in ApoE-Deficient Mice: S.W. Huang, et al.; Thromb. Haemost.
118, 401 (2018),
Abstract;
Advanced age in mares affects endometrial secretion of arachidonic acid metabolites during equine subclinical endometritis: M.J. Siemieniuch, et al.; Theriogenology
103, 191 (2017),
Application(s): ELISA using culture supernatants,
Abstract;
Colon cancer-derived myofibroblasts increase endothelial cell migration by glucocorticoid-sensitive secretion of a pro-migratory factor: Z. Drebert, et al.; Vascul. Pharmacol.
89, 19 (2017),
Application(s): Determine the concentrations of prostanoids in conditioned medium from myofibroblasts and HUVECs, and in HUVEC lysates,
Abstract;
Effect of Regular Aerobic Activity in Young Healthy Athletes on Profile of Endothelial Function and Platelet Activity: K. Podgórska, et al.; BioMed Res. Int.
2017, 8715909 (2017),
Application(s): Human plasma,
Abstract;
Full Text
Protective Effects of Total Flavones of Elaeagnus rhamnoides (L.) A. Nelson against Vascular Endothelial Injury in Blood Stasis Model Rats: Z. Wei, et al.; Evid. Based Complement. Alternat. Med.
2017, 8142562 (2017),
Abstract;
Full Text
Altered secretion of selected arachidonic acid metabolites during subclinical endometritis relative to estrous cycle stage and grade of fibrosis in mares: K. Gajos, et al.; Theriogenology
84, 457 (2015),
Application(s): ELISA using mare sample,
Abstract;
Effect of Cyclooxygenase(COX)-1 and COX-2 inhibition on furosemide-induced renal responses and isoform immunolocalization in the healthy cat kidney: L. Pelligand, et al.; BMC Vet. Res.
11, 296 (2015),
Abstract;
Full Text
Endothelial dysfunction in young healthy men is associated with aspirin resistance: A. Doroszko, et al.; Vascul. Pharmacol.
67-69, 30 (2015),
Application(s): ELISA using human blood,
Abstract;
Urea-induced ROS cause endothelial dysfunction in chronic renal failure: M. D'Apolito, et al.; Atherosclerosis
239, 393 (2015),
Application(s): ELISA,
Abstract;
Carnosol and carnosic acids from Salvia officinalis inhibit microsomal prostaglandin E2 synthase-1: J. Bauer, et al.; J. Pharmacol. Exp. Ther.
342, 169 (2012),
Application(s): ELISA using human cell culture supernatants,
Abstract;
Arzanol, a prenylated heterodimeric phloroglucinyl pyrone, inhibits eicosanoid biosynthesis and exhibits anti-inflammatory efficacy in vivo: J. Bauer, et al.; Biochem. Pharmacol.
81, 259 (2011),
Application(s): ELISA using human cell culture supernatants,
Abstract;
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