Popular ELISA
|
Enzo Life Sciences offers hundreds of ELISA kits in both immunometric and competitive assay formats. As scientists and manufacturers of kits, we understand the critical nature of your research.
Each enzyme-linked immunosorbent assay is put through rigorous testing to ensure high precision, accuracy, sensitivity, and specificity. You can be confident that you will obtain reproducible results, day-after day and lot-after-lot.
Enzyme-linked immunosorbent assay (ELISA) is an analytical, plate-based technique to detect and quantify the presence of an target analyte in a liquid sample using antibodies.
Assay kits can be convenient time-saving tools to help accelerate your research, but not all assays are created equal. Some that promise convenience fail to deliver biologically relevant sensitivity, or the reproducibility needed for long-term studies. This can cost you more time and money in the end. Whether you use an ELISA assay for biomarker detection, assess cellular function with fluorescent probes, or screen enzyme modulators with activity assays, every Enzo assay kit includes something a lot of other companies out there today can’t -- Experience.
It takes more than just an antibody pair to make an ELISA assay. Building an immunoassay requires screening multiple antibodies, selection of appropriate standards and conjugates, establishing proper sample prep protocols, and validation of the assay in relevant matrices. Our expertise in developing ELISAs is further backed by two decades of manufacturing excellence. Strict validation criteria and state-of-the-art manufacturing facilities deliver reproducible assays that continue to be cited in peer-reviewed publications by scientists around the world.
Reliable Manufacturing Ensures Consistent Results
The graph above demonstrates the robust nature of our immunoassays, showing standard curves for 7 lots of our competitive ELISA for cyclic AMP manufactured over 3 years.
| "For decades, Enzo’s ELISAs have been trusted by researchers. We have long been a manufacturer of consistent, reliable, functional immunoassays that deliver relevant results at a cost-effective price per sample. | Maria Pulina Product Manager |
|
Highly Cited. Highly Trusted.
Enzo’s ELISA Kits have been cited more than 1800 times! You can find the citations on each product page. |
Immunometric assays, also known as sandwich ELISAs (enzyme-linked immunosorbent assay), use two antibodies specific to the antigen to capture or "sandwich” antigen in the well for detection. Immunometric assays exhibit a direct correlation between antigen concentration and substrate response. Immunometric assays typically employ a "capture” antibody coated on the plate to bind the antigen of interest. During a second incubation, the antigen is bound by a second "detection” antibody that is also specific to the antigen. The detection antibody can either be bound by a secondary antibody-enzyme conjugate, or the detection antibody itself is enzyme-conjugated. When chromogenic substrate is added to the assay to develop color, samples with high antigen concentration generate more signal than those with low antigen concentration, producing a signal directly proportional to the amount of antigen in the sample. This correlation can then be used to extrapolate the concentration of antigen in an unknown sample from a standard curve.
In competitive enzyme immunoassays (EIA) the antigen in a sample competes for limited antibody binding sites with antigen conjugated to a reporter enzyme. This produces an inverse relationship between antigen concentration and substrate turnover. Competitive EIAs typically use a single antibody to a small molecular weight antigen, generally less than 10,000 Daltons. During incubation, samples with high antigen content result in unlabeled antigen being bound in greater amounts than conjugated antigen. When chromogenic substrate is added to the assay to develop color, samples with high antigen concentration generate a lower signal than those containing low antigen concentration, yielding the inverse correlation between antigen concentration in the sample and color development in the assay. This relationship can then be used to extrapolate antigen concentration in an unknown sample from a standard curve. This type of reaction is one of the few methods possible for small molecular weight antigens, such as steroids, drugs, lipids and peptides.
|
Enzo offers research kits, biochemicals and biologicals backed by over 40 years of expertise. As Scientists Enabling Scientists™, we realize the value in providing relevant information to our customers working in the fields of life sciences, drug development and clinical research. We are happy to provide simple but useful tips for improving daily tasks as well as the overall quality of your research.
|
 |
|
Bioprocess ELISA Kits |
Cyclic Nucleotides ELISA Kits |
Inflammation ELISAs |
Heat Shock Response ELISA Kits |
|||
|
Oxidative Stress ELISA Kits |
Toxicology ELISA Kits |
Steroid / Hormone ELISA kits Sensitive ELISAs for the detection of steroid and hormones. |
Wnt Pathway ELISA Kits |
Oxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hypothalamus and stored in the posterior pituitary. A hormone once thought to be limited to female smooth muscle reproductive physiology, more current findings have determined that oxytocin also functions as a neurotransmitter, may be involved in neuropsychiatric disorders, social/sexual behavior and is important in male reproductive physiology. Oxytocin and the related neurohypophysial peptide, Arg8-Vasopressin, maintain renal water and sodium balance.
With thousands of species each year listed as endangered or threatened, the work done by biologists around the world to understand and preserve animal health is never ending.
Reproduction studies and stress are two of the most common concerns today. Hormone analysis continues to be widely accepted for monitoring stress response and evaluating reproductive health on wildlife.
Enzo Life Sciences provides a broad range of immunoassay kits for monitoring stress, reproductive physiology, health and welfare, and survival of wildlife animals.
Featured ELISA Assays
Stress Monitoring: Corticosterone ELISA Kit
Reproductive Studies: Testosterone ELISA Kit
Reliable detection of analytes that are present at low concentrations is a critical need for early diagnosis and treatment of various diseases. Enzo Life Sciences has developed a highly sensitive ELISA Amplification System to be used with your most difficult-to-detect markers.
Are Sample Extraction Methods Necessary For ELISA?
2020 October 9 | Tags:
Successful Research Tips
Which Controls to Use in ELISA Assays?
November 7 | Tags:
Successful Research Tips
Why are Sensitivity and Specificity Important Parameters for an ELISA?
2019 September 13 | Tags:
Successful Research Tips
Why is ELISA lot-to-lot Reproducibility Critical for your Research?
2019 August 2 | Tags:
Successful Research Tips
How to Validate Sample Dilutions and Achieve Linearity in New Sample Types
2018 October 12 | Tags:
Successful Research Tips
%CV in ELISA: How to Reduce Them and Why They’re Important
2018 January 19 | Tags:
Successful Research Tips
What are the differences between ELISA assay types?
2017 April 28 | Tags:
Successful Research Tips
10 Tips for Successful ELISA Assay
2015 May 29 | Tags:
Successful Research Tips,
We Are Enzo!
