ProteoStat® Thermal Shift Stability Assay

Product Performance
ProteoStat® Dye Mechanism
Instrumentation
Applications

ProteoStat® Thermal Shift Stability Assay Flyer

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Producing proteins and peptides for drugs and research is time consuming and expensive. Care must be taken during production, storage and use of the protein to ensure that it remains stable and active. One of the most common problems involved in the storage of proteins and peptides is the formation of aggregates over time. It is desirable to implement a method that can rapidly screen conditions that may help stabilize a protein and prevent aggregation.

The ProteoStat® Thermal Shift Stability Assay is a rapid, simple screening method based on a dye that detects protein aggregates. The assay does not require any prior knowledge of protein structure, sequence or ligand binding activity. This dye in the assay is based on a molecular rotor that has very low fluorescence in the absence of aggregated proteins, but fluoresces brightly when bound to the surface of protein aggregates.

• High-throughput thermal shift assay for determining the impact of ligand-protein interactions on target protein stability
• Suitable for accelerated analysis of parameters impacting monoclonal antibody and recombinant protein stability (pH, ionic strength, cryoprotectants, excipients)
• Measures aggregation temperature, providing more flexibility than assays using environment-sensitive dyes to measure unfolding temperature, where detergent and hydrophobic compound interference can be problematic
• Assay is readily performed using a wide range of thermally-controlled fluorescence reading devices (Fluorimeters or real-time PCR instruments)