United States
FluoForte Calcium Assay is Visibly Brighter
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FluoForte™ Calcium Assay Kit
Utilizing the brightest and most sensitive fluorescent Ca2+ indicator, FluoForte™ Calcium Assay kit provides much higher signal intensity and the largest assay window on the market. Enzo Life Sciences' homogeneous fluorescence-based assay kit detects calcium mobilization across a broad spectrum of biological targets. Easy-to-use protocol does not require a wash step or exogenous addition of a quencher dye, which could adversely affect receptor-ligand interaction kinetics. The assay can be performed in a convenient 96-well or 384-well microplate format and easily adapts to automation.
FluoForte™ dye offers superior measurement of challenging cell lines and receptors; unlike other widely used calcium ion indicators in high-throughput screening applications; Fluo-3, Fluo-4 and Calcium 4 dyes have relatively weak fluorescence signals limiting their application in these instances.
Product family: Dyes, Stains, Fluorescent Probes
Features | Benefits |
|---|---|
Load dye in live cells at 37°C or RT | Room temperature is more compatible with HTS applications |
Homogeneous mix-and-read, no-wash calcium mobilization assay | Washing cells introduces errors |
Significantly brighter fluorescence intensity | 2X brighter than Fluo-4, Fluo-4 Direct, Fluo-4 NW, FLIPR® Calcium 4 assay kits 4X brighter than Fluo-3 |
EC50 values (half maximal effective concentration) | Comparable with Fluo-3, Fluo-4, Fluo-4 Direct™ and FLIPR® Calcium assay kits |
No quencher dye | No potential interference from compound ligand-receptor interactions |
Larger assay window than other dyes | Easily detects low calcium mobilization in challenging cell lines and receptors |
Maximum excitation @ ~490 nm | Readily adopted into established protocols and instrument workflows |
Stringently manufactured | Controls and eliminates non-specific assay artifacts |
