United States
Organelle detection kits
Cells do not appear to be healthy
- Some cells require serum to remain healthy >> Add serum to the staining solution. Serum does not affect staining. Normal amounts of serum added range from 2% to 10%.
Organelles are not sufficiently stained
- Very low concentration of dye was used or dye was incubated with the cells for an insufficient length of time >> Increase the labeling concentration or increase the time allowed for the dye to accumulate in the organelle.
Blue nuclear counter-stain is too bright compared to the organelle stain
- Different microscopes, cameras and filters may make some signals appear very bright >> Reduce the concentration of the nuclear counter-stain or shorten the exposure time.
The dyes fail to stain acidic organelles in fixed and/or permeabilized cells.
- The following kits are only suitable for live cell staining only >> use only for live cell analysis:
- Cyto-ID Autophagy detection kit
- Lyso-ID Red or Green lysosomal detection kit
- Lyso-ID Red Cytotoxicity kit
- Mito-ID Membrane Potential kits
- Nuclear-ID Green/Red nucleoli / nuclei detection kit
- Nucleolar-ID Green nucleoli detection kit
- Organelle-ID RGB III assay kit
