Multidrug Resistance Assay Kits

CELLestial™ Kits Trouble Shooting
CELLestial™ Dyes & Kits FAQs

Technical Support Request Form

Cells do not exhibit fluorescence after incubation with the detection reagent.

• Cell viability is low >>
  • Cells should be in log growth phase.
  • Cell samples cannot be kept longer than 6 hours before the assay.
  • Do not use fixatives, azides or other preservatives.
  • Avoid shear stress, do not vortex, and avoid bubbling .
• Very low concentration of the eFluxx-ID™ Detection Reagent >> Check the concentration of the reagent.
  
  

Fluorescence does not increase after incubation with inhibitor

• Cells do not express any MDR1, MRP1/2 or BCRP >> Use a positive control cell type expressing corresponding ABC transporter.
  
  

There are differences in MFI (mean fluorescence intensity) values between cell lines

• Detection reagent accumulation may be influenced by cell size, endogenous esterase activity, etc. >> Using the inhibitors and calculating the MAF values eliminate these differences.
  
  

Inconsistent fluorescence shift using the same cell (irreproducible results)

• Inadequate incubation condition >> Always use a water bath (not incubator) and ensure temperature of water bath is 37°C