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Complete Toolbox For Your Epigenetics Research

Posted By Arun Kumar
Tags: Epigenetics

Epigenetics is the structural adaptation of chromosomal regions to register, signal, or perpetuate altered activity states. Alterations to chromatin via direct DNA modification (e.g., methylation) or post-translational modification (PTM) of histones (e.g., acetylation, methylation, and ubiquitination) affect gene transcription. To date, acetylation/deacetylation of histone lysine residues and methylation of DNA have proven to be of greatest clinical significance amongst epigenetic modifications. Recent approval of anti-cancer drugs such as Vorinostat and Decitabine, which target histone deacetylases (HDACs) and DNA methyltransferases (DNMTs), respectively, is evidence that reprogramming gene expression on an epigenetic level holds great clinical promise. Drugs targeting critical PTM-regulating enzymes continue to populate pharma development pipelines.


For over a decade, Enzo Life Sciences’ Epigenetics platform has revolutionized the use of HDAC & Sirtuin enzyme activity kit assays, freeing researchers from cumbersome protocols required with radio-labeled or other modified histone-based methods. Our high-quality assays utilize patented substrate/developer chemistry in combination with high-activity, high-purity enzymes, to deliver higher quality hits. Broad class HDAC/Sirtuin screening assays are available in fluorescent, colorimetric, and chemiluminescent formats.




FLUOR DE LYS® HDAC & Sirtuin Assays are convenient broad class screening formats. HDAC activity varies due to different expression levels based on cell types. Therefore, cell-based HDAC experiments are especially relevant for their ability to address the natural context of HDAC enzymes. We offer many targeted enzyme-specific HDAC/Sirtuin assay kits. Check out our FLUOR DE LYS Substrates and Accessories for more information on our substrates, standards, developers, and nuclear extract.

Flexible HDAC & Sirtuin Assays

  • Non-radioactive, homogeneous, HTS compatible formatsv
  • Green & chemiluminescent assays available to minimize interference
  • Assay all HDAC classes from multiple sources
  • High-activity, in-house manufactured control enzymes
  • Widely cited in peer-reviewed literature

GENERAL HDAC/SIRTUIN ASSAY KITS
Product Name Size
FLUOR DE LYS® HDAC Cellular Activity Assay Kit 96 Reactions
FLUOR DE LYS® HDAC Activity Assay Kit 96 Reactions
FLUOR DE LYS® Green HDAC Activity Assay Ki 96 Reactions
GENERAL HDAC/SIRTUIN ASSAY KITS
Product Name Size
FLUOR DE LYS® HDAC1 Activity Assay Kit 96 Reactions
FLUOR DE LYS® HDAC2 Activity Assay Kit 96 Reactions
FLUOR DE LYS® HDAC3/NCOR1 Activity Assay Kit 96 Reactions
FLUOR DE LYS® HDAC6 Activity Assay Kit 96 Reactions
FLUOR DE LYS® HDAC8 Activity Assay Kit 96 Reactions
FLUOR DE LYS® SIRT1 Drug Discovery Kit 96 Reactions
FLUOR DE LYS® SIRT2 Drug Discovery Kit 96 Reactions
FLUOR DE LYS® SIRT3 Drug Discovery Kit 96 Reactions
FLUOR DE LYS® SIRT5 Drug Discovery Kit 96 Reactions
FLUOR DE LYS® Green SIRT5 Drug Discovery Kit 96 Reactions



COLOR DE LYS® HDAC colorimetirc activity assay kit (Colorimetric Histone de Acetylase Lysyl Substrate/Developer) is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. Our COLOR DE LYS® assay is designed to measure HDAC activity in cell or nuclear extracts, immunoprecipitates, or purified enzymes. The included HeLa nuclear extract can be used as a positive control, or as a source of HDAC1 and 2 for use in inhibitor screening.
  • Simple two-step protocol with < 1 hour time to results
  • Colorimetric readout at 405 nm compatible with most plate readers
  • Resistant to detergent interference common to antibody-based assays
  • Eliminate need for radioactivity, extractions, and/chromatography
  • Suitable for high-throughput analysis


Reaction scheme of the HDAC Colorimetric Activity Assay (patent pending). Deacetylation of the substrate sensitizes it to the developer, which causes an increase in yellow intensity and absorption at 405 nm.




CHEMILUM DE LYS® HDAC/SIRT Chemiluminescent Drug Discovery Kit is the best option for false negatives or positives resulting from the effects of compound(s) being screened on elements of the report as summarized below. Based on our CHEMILUM DE LYS® substrate and developer combination, the HDAC/SIRT Chemiluminescent Drug Discovery kit provides a chemiluminescent alternative to radiolabeled and HPLC methods for HDAC activity. Compared to traditional luciferase and fluorescence-based screening assays for HDAC and Sirtuin activity, this platform offers a 3-step assay procedure designed for cellular extracts, immunoprecipitates, or purified extracts.
  • Eliminates interference seen in Luciferase-based assays
  • Superior signal-to-noise ratio with no interference from cell extract detergents
  • Consistent results from a validated system

Eliminate False HDAC/SIRT Screening Hits with CHEMILUM DE LYS®

Competitor HDAC Assays
CHEMILUM DE LYS® Luciferase-Based Chemiluminescent Assay Colorimetric Assay Fluorometric Assay
No Artifactual Activation by Resveratrol
Resistant to Protease Inhibitors
Resistant to Kinase Inhibitors



Enzo Life Sciences provides a comprehensive portfolio of widely cited active HDAC and Sirtuin proteins, HATS, methyltransferases and demethylases. Our SCREEN-WELL® Epigenetics library contains 43 compounds with defined activity against epigenetics-regulating enzymes, each of which can be supplied individually upon request. Our portfolio of reagents also includes antibodies for the detection of key epigenetics-regulating enzymes and substrates, including modification-specific antibodies for acetylated, methylated, phosphorylated or ubiquitinated epitopes. Further details can be found on our Epigenetics platform page. Please contact our Technical Support Team for further assistance and check out our TechNotes, an easy way for you to see how Enzo Life Sciences is living up to our motto: Scientists Enabling Scientists™.


GENERAL HDAC/SIRTUIN ASSAY KITS
Product Name Size
HDAC (rat liver) 2 mL
HDAC1 (human) (recombinant) (His-tag) 50 μg
HDAC2 (full-length) (human) (recombinant) (His-tag) 50 μg
HDAC2 (human) (1-488) (recombinant) (His-tag) 50 μg
HDAC3 (human) (recombinant) (His-tag) 50 μg
HDAC3 / NCOR1 complex (human) (recombinant) 50 μg
HDAC6 (human) (recombinant) (His-tag) 50 μg
HDAC8 (human) (recombinant) 100 U
HDAC10 (human) (recombinant) (His-tag) 50 μg
HDAC11 (human) (recombinant) (His-tag) 50 μg
SIRT1 (human) (recombinant) (His-tag) 100 U
SIRT2 (human) (recombinant) (His-tag) 500 U
SIRT3 (human) (recombinant) (His-tag) 500 U
SIRT5 (human) (recombinant) (His-tag) 50 KU
GENERAL HDAC/SIRTUIN ASSAY KITS
Product Name Size
FLUOR DE LYS® Deacetylase Substrate 50 μL
FLUOR DE LYS® H4-AcK16 Deacetylase Substrate 0 .5 μmol
FLUOR DE LYS® HDAC8 Deacetylase Substrate 0 .5 μmol
FLUOR DE LYS® SIRT1 Deacetylase Substrate 0 .5 μmol
FLUOR DE LYS® SIRT2 Deacetylase Substrate 0 .5 μmol
FLUOR DE LYS®-Green Substrate 50 μL
FLUOR DE LYS®-Succinyl, Desuccinylase Substrate 50 μL
FLUOR DE LYS®-Succinyl Green, Desuccinylase Substrate 50 μL
FLUOR DE LYS® Developer Concentrate 300 μL
FLUOR DE LYS® Developer II 1 .25 mL

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SUBSTRATE PREFERENCES FOR HDAC AND SIRTUIN ENZYMES
Substrate HDAC Sirtuin
1 2 3 6 8 10 11 HeLa Nuclear Extract 1 2 3 5
FLUOR DE LYS® Deacetylase Substrate +++ ++ ++++ ++ + ++++ ++ ++ + + + +
FLUOR DE LYS® H4-AcK16 +++ +++ ++++ ++++ +++ n.d. +++ +++ ++++ +++ + +++
FLUOR DE LYS® HDAC8 ++++ +++++ ++++ +++++ +++++ n.d. +++++ +++++ +++ ++++ +++ ++++
FLUOR DE LYS® SIRT1 +++++ +++++ ++++ +++++ +++++ n.d. +++++ +++++ +++ ++++ +++ +++++
FLUOR DE LYS® SIRT2 +++ ++++ ++++ ++++ ++ + + ++++ ++ +++++ +++++ +++++
FLUOR DE LYS® Substrate Concentration 5μM 5μM 50μM 50μM 25μM 25μM 5μM 25μM 25/50μM 25μM 10μM 500μM

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