Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Determining HER Genes Alterations May Provide New Tools for Kidney Cancer Classification

Posted By Jan-Henning Martens
Tags: Cancer, Molecular Biology, Screening

Renal cell carcinoma (RCC) is the most common type of kidney cancer in adults, responsible for approximately 90% of cases. The American Cancer Society’s most recent estimates for kidney cancer in the United States for 2016 are that 62,700 new cases of kidney cancer (39,650 in men and 23,050 in women) will occur and that 14,240 people (9,240 men and 5,000 women) will die from this disease. Kidney cancer is among the ten most common cancers in both men and women. Overall, the lifetime risk for developing kidney cancer is 1.6% and this risk is higher in men than in women. Interestingly, kidney cancer is very uncommon in people aged below 45 with the average diagnostic age being 64. It is also noteworthy that the rate of new kidney cancers has been rising since the 1990s with little improvement on developing successful treatments.

Chromophobe renal cell carcinoma (chRCC) is the third most common subtype of kidney cancer and accounts for 5% of all RCC cases. The five year disease free survival rate of chRCC is reported to be increased compared with that of other RCC subtypes, including clear cell, sarcomatoid and papillary renal cell carcinoma. Although the outcomes of chRCC are typically more favorable compared with those of other subtypes, the disease still demonstrates a 6 7% probability of tumor progression and metastasis. Cytogenetic analysis revealed an association between chRCC and the loss of chromosomes 1, 2, 6, 10, 13, 17 and 21; therefore, such losses may be prominent abnormalities useful for the diagnosis of the disease. In addition, differential gene expression has been used to assist in the diagnosis of chRCC and pathway analyses emphasized the involvement of Erb‑B2 receptor tyrosine kinase 2 [human epidermal growth factor receptor (HER) 2] signaling in chRCC.

The ErbB/HER family of proteins contains four receptor tyrosine kinases, structurally related to the epidermal growth factor receptor (EGFR/HER1), its first discovered member. In humans, the family includes HER1 (EGFR, ErbB1), HER2 (Neu, ErbB2), HER3 (ErbB3), and HER4 (ErbB4). Insufficient ErbB/HER signaling in humans is associated with the development of neurodegenerative diseases, such as multiple sclerosis and Alzheimer's disease. Whereas, excessive ErbB/HER signaling is associated with the development of a wide variety of solid tumors. Lung and colorectal cancers and glioblastoma may develop because of aberrant Her1 signaling while breast cancer is often associated with excessive Her2 activation. All four members of the ErbB/HER protein family are capable of forming homodimers, heterodimers, and possibly higher-order oligomers upon binding to the 11 different growth factors that are known to activate ErbB/HER receptors.

Wen Hui Weng and colleagues from National Taipei University of Technology and Chang Gung Memorial Hospital and University analyzed 11 chRCC specimens from patients diagnosed between 2005 and 2009 by fluorescence in situ hybridization (FISH) for HER family gene abnormalities. The required probes for all target genes were prepared by nick translation labeling of several bacterial artificial chromosomes (BACs) using Enzo’s Green and Red dUTPs. FISH-stained samples were analyzed by fluorescence microscopy. These investigations showed that the loss of one copy of the HER2 and HER4 genes was the major alteration of the tumor cells in all chRCC cases. Statistical data also indicated that loss of the HER2 gene was strongly correlated with loss of the HER4 gene and amplification of HER1 was also strongly correlated with the amplification of HER4. Furthermore, a high percentage of genetic structural rearrangements was observed in HER3 genes, which was significantly associated with amplification of HER2. These findings indicate that further characterization of the underlying aberrant functions of HER genes may be of considerable interest for additional studies in the context of using HER genes to distinguish between RCC subtypes and to establish improved treatment guidelines.

From our superior labeling technology for microarrays to labeling systems, probes and detection assays for (fluorescent) in situ hybridization, Enzo Life Sciences provides a complete set of genetic tools, some of which are described below. Enzo Life Sciences will also be attending ESHG 2016 in Barcelona, May 21-24. Come find us at our booth to celebrate our 40th year anniversary and learn more about our Genomics product range.

Share this TechNote

Never miss a new TechNote!

Receive our TechNotes as soon as they are published.


Follow Us!

 
comments powered by Disqus

References:

  1. W. H. Weng, et al. Genetic alterations of HER genes in chromophobe renal cell carcinoma. Oncol Lett. (2016) 11: 2111.

Related Products

CYTAG® CGH Labeling kit 

Superior labeling efficiency and better dye incorporation results in less failed runs
CGH | Print as PDF
 
ENZ-42671-K010 2x10 Reactions Inquire for pricing
 
ENZ-42671-K100 2x100 Reactions Inquire for pricing
Do you need bulk/larger quantities?
 

CYTAG® TotalCGH Labeling kit 

Complete kit to digest and label DNA for CGH+SNP arrays
CGH | Print as PDF
 
ENZ-42674-0010 2x10 Reactions Inquire for pricing
 
ENZ-42674-0100 2x100 Reactions Inquire for pricing
Do you need bulk/larger quantities?
 

CGH labeling kit for BAC arrays 

CGH | Print as PDF
 
ENZ-42670 20 Reactions Inquire for pricing
Do you need bulk/larger quantities?
 

BIOSCORE® Screening and amplification kit (20 reactions) 

Print as PDF
 
ENZ-42440 1 Kit 490.00 USD
Do you need bulk/larger quantities?
 

BIOARRAY™ Single-round RNA amplification and biotin labeling system 

Improved data quality and  analysis results through greater biotin incorporation.
Print as PDF
 
ENZ-42420-10 10 Reactions 805.00 USD
Do you need bulk/larger quantities?
 

BIOARRAY HIGHYIELD® RNA transcript labeling kit (T7) 

The gold standard in transcript labeling, for use with Affymetrix® GeneChip® microarrays.
Print as PDF
 
ENZ-42655-10 10 Reactions 845.00 USD
 
ENZ-42655-40 40 Reactions 2,645.00 USD
 
ENZ-42655-100 100 Reactions 4,955.00 USD
Do you need bulk/larger quantities?
 

BIOARRAY™ Low Input RNA Amplification and Biotin Labeling System 

Lower RNA Input with High Yield – High Quality Biotin-labeling
Print as PDF
 
ENZ-42422-0010 10 Reactions 758.00 USD
Do you need bulk/larger quantities?
 

FLOWSCRIPT® HPV E6/E7 assay kit 

Flow cytometry-based assay for the single cell detection of mRNAs for HPV oncoproteins E6 and E7
Flow Cytometry, Fluorescent detection | Print as PDF
 
ENZ-GEN300-0100 100 tests Inquire for pricing
Do you need bulk/larger quantities?
 

PATHO-GENE® HPV screening probe 

Print as PDF
 
ENZ-32884-6000 6 ml Inquire for pricing
 
ENZ-32884 1 ml 648.00 USD
Do you need bulk/larger quantities?
 

PATHO-GENE® HPV type 16/18/31/33/51 probe 

HPV probe that can be optimized for use with polymer detection system or used as optimized RTU with Enzo's Streptavidin detection methods.
Print as PDF
 
ENZ-32882-6000 6 ml Inquire for pricing
 
ENZ-32882-1000 1 ml 648.00 USD
Do you need bulk/larger quantities?
 

PATHO-GENE® HPV type 31/33/51 probe 

A mixture of biotin-labeled HPV 31, HPV 33 and HPV 51-specific probes for in situ hybridization
Print as PDF
 
ENZ-32887-6000 6 ml Inquire for pricing
 
ENZ-32887 1 ml 541.00 USD
Do you need bulk/larger quantities?
 

PATHO-GENE® HPV type 16/18 probe 

A mixture of biotin-labeled HPV 16 and HPV 18-specific probes for in situ hybridization
Print as PDF
 
ENZ-32886-6000 6 ml Inquire for pricing
 
ENZ-32886 1 ml 541.00 USD
Do you need bulk/larger quantities?
 

PATHO-GENE® HPV type 6/11 probe 

A mixture of biotin-labeled HPV 6 and HPV 11-specific probes for in situ hybridization
Print as PDF
 
ENZ-32885-6000 6 ml Inquire for pricing
 
ENZ-32885 1 ml 852.00 USD
Do you need bulk/larger quantities?
 

PATHO-GENE® AP-NBT/BCIP HPV in situ screening assay 

Colorimetric detection | Print as PDF
 
ENZ-32879 20 assays 1,403.00 USD
Do you need bulk/larger quantities?
 

PATHO-GENE® HRP-AEC HPV in situ typing assay (types 6/11, 16/18 and 31/33/51) 

Colorimetric detection | Print as PDF
 
ENZ-32877 20 assays 2,550.00 USD
Do you need bulk/larger quantities?
 

BIO-PROBE® Nick translation kit with fluorescein-12-dUTP 

Dot Blot, ISH (in situ hybridization), Northern Blot, Southern Blot | Print as PDF
 
ENZ-42710-16 25 Reactions 355.00 USD
Do you need bulk/larger quantities?
 

Nick translation DNA labeling system 

Nick translation  system for preparing FISH probes
FISH | Print as PDF
 
ENZ-42910 50 Reactions 288.00 USD
Do you need bulk/larger quantities?
 

ULTRA SENSITIVE® Enhanced Hrp-DAB in situ detection system 

Colorimetric detection | Print as PDF
 
ENZ-32400 30 assays 220.00 USD
Do you need bulk/larger quantities?
 

Digoxigenin-dUTP, alkali-stable 

Digoxigenin-labeled nucleotide suitable for non-radioactive labeling of DNA probes
≥93% (HPLC), ISH (in situ hybridization), Southern Blot | Print as PDF
 
ENZ-NUC113-0025 25 nmol 180.00 USD
Do you need bulk/larger quantities?
 

Digoxigenin-UTP, alkali-stable 

Digoxigenin-labeled nucleotide suitable for non-radioactive labeling of RNA probes
≥93% (HPLC), ISH (in situ hybridization), Northern Blot | Print as PDF
 
ENZ-NUC114-0250 250 nmol 210.00 USD
Do you need bulk/larger quantities?
 

Aqua 431 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42853 25 nmol 318.00 USD
Do you need bulk/larger quantities?
 

Green 500 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42845 25 nmol 250.00 USD
Do you need bulk/larger quantities?
 

Gold 525 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42843 25 nmol 318.00 USD
Do you need bulk/larger quantities?
 

Orange 552 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42842 25 nmol 300.00 USD
Do you need bulk/larger quantities?
 

Red 650 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42522 25 nmol 315.00 USD
Do you need bulk/larger quantities?
 

Bio-16-dUTP 

≥93% (HPLC) | Print as PDF
 
ENZ-42811 50 nmol 375.00 USD
Do you need bulk/larger quantities?
 

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,