Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

FISHing for the right probe

Fluorescence in situ hybridization (FISH) is a cytogenetic technique used to detect the absence/presence and location of specific DNA/RNA sequences. This technique allows the microscopic visualization of abnormalities such as chromosomal deletion, amplification and translocation/inversion. Pathologists can select a specific area of a tumor to be examined by FISH and directly correlate FISH results obtained under fluorescence or confocal microscopy and tumor morphology observed under conventional light microscopy. FISH is currently used for prenatal diagnosis as well as a diagnostic and prognostic tool for various cancer types. Successful FISH requires optimization of several elements including tissue fixation and permeabilization, design of the fluorescently-labeled target-specific nucleic acid probes, and their annealing and detection within the tissue of interest. Despite the development and the availability of different RNA FISH methods, specialists struggled with low levels of fluorescence when using formalin-fixed and paraffin-embedded (FFPE) tissue samples. Recently, Dr. Renwick and colleagues from the Rockefeller University demonstrated that the poor quality of the results was not due to RNA denaturation occurring during fixation and embedding of the tissue sample but to the lack of optimization done on the probes. Refined and enhanced fluorescence signals were obtained by increasing probe-hapten linker length and shortening probes to minimize ribosomal RNA sequence complementarity. The researchers showed the utility of this optimization when detecting novel microRNAs (miRNAs) that could differentiate between two skin cancers, miR-205 in basal cell carcinoma (BCC) and miR-375 in Merkel cell carcinoma (MCC). As a proof of concept, they designed probes against these two miRNAs, normalized the signals obtained against directly detectable reference ribosomal RNA signals and successfully identified tumors in blinded analysis. This technique should be widely applicable to all miRNAs in any FFPE tissue. However, it remains to be seen whether this technique works in a larger sample collection before implementing it as a routine test in clinical diagnosis laboratory.

Enzo Life Sciences offers a comprehensive portfolio for FISH and ISH analysis including labeling, typing and detection assays, deoxynucleotides, dideoxynucleotides and ribonucleotides, some of which are described below:

Share this TechNote

Follow Us!

 
comments powered by Disqus

References:

Related Products

PATHO-GENE® HRP-AEC HPV in situ typing assay (types 6/11, 16/18 and 31/33/51) 

Colorimetric detection | Print as PDF
 
ENZ-32877 20 assays 2,550.00 USD
Do you need bulk/larger quantities?
 

PATHO-GENE® AP-NBT/BCIP HPV in situ screening assay 

Colorimetric detection | Print as PDF
 
ENZ-32879 20 assays 1,431.00 USD
Do you need bulk/larger quantities?
 

Rabbit anti-biotin linker (Ready-to-use) 

Partially purified IgG fraction of rabbit serum., ISH (in situ hybridization) | Print as PDF
 
ENZ-32892-6000 6 ml 385.00 USD
Do you need bulk/larger quantities?
 

Mouse anti-biotin linker (Ready-to-use) 

ISH (in situ hybridization) | Print as PDF
 
ENZ-32893-6000 6 ml 374.00 USD
Do you need bulk/larger quantities?
 

Cyanine 3-dUTP 

≥93% (HPLC) | Print as PDF
 
ENZ-42501 25 nmol 179.00 USD
Do you need bulk/larger quantities?
 

Cyanine 5-dUTP 

≥93% (HPLC) | Print as PDF
 
ENZ-42502 25 nmol 179.00 USD
Do you need bulk/larger quantities?
 

Cyanine 3-UTP (enhanced) 

≥93% (HPLC) | Print as PDF
 
ENZ-42505 250 nmol 316.00 USD
Do you need bulk/larger quantities?
 

Cyanine 5-UTP (enhanced) 

≥93% (HPLC) | Print as PDF
 
ENZ-42506 250 nmol 316.00 USD
Do you need bulk/larger quantities?
 

Gold 550 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42521 25 nmol 321.00 USD
Do you need bulk/larger quantities?
 

Red 650 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42522 25 nmol 321.00 USD
Do you need bulk/larger quantities?
 

Green 496 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42831 25 nmol 255.00 USD
Do you need bulk/larger quantities?
 

Fluorescein-12-ddUTP 

≥93% (HPLC) | Print as PDF
 
ENZ-42833 25 nmol 317.00 USD
Do you need bulk/larger quantities?
 

Fluorescein-12-UTP 

≥93% (HPLC) | Print as PDF
 
ENZ-42834 250 nmol 324.00 USD
Do you need bulk/larger quantities?
 

Orange 552 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42842 25 nmol 306.00 USD
Do you need bulk/larger quantities?
 

Gold 525 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42843 25 nmol 324.00 USD
Do you need bulk/larger quantities?
 

Red 580 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42844 25 nmol 324.00 USD
Do you need bulk/larger quantities?
 

Aqua 431 dUTP 

≥93% (HPLC), FISH | Print as PDF
 
ENZ-42853 25 nmol 324.00 USD
Do you need bulk/larger quantities?
 

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2018 Enzo Life Sciences, Inc.,