Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

Nobel Prize Winners Experience GPCR-Mediated Adrenaline Rush

You can imagine that accepting a Nobel Prize in Chemistry would provide an amazing sense of pride and accomplishment, a true adrenaline rush. But would that experience feel any different if the very research you were being recognized for was in part responsible for our understanding of how such feelings and emotions arise from biochemical signals, such as those transmitted via G-Protein Coupled Receptors (GPCRs)? You’d have to ask Robert J. Lefkowitz and Brian K. Kobilka to know for sure. This past December, Lefkowitz (Duke University) and Kobilka (Stanford University) were awarded the 2012 Nobel Prize for Chemistry for their contributions to unfolding the structure and function of GPCRs.

GPCRs belong to a very large family of proteins involved in the transmission of intra-cellular signals following the binding of ligands (including hormones, neurotransmitters and cytokines) to their extra-cellular domains and the recruitment of intra-cellular GTP in order to activate or inhibit enzymes such as adenylate cyclase or phospholipase C. Several cell surface receptors were characterized by Lefkowitz’s laboratory using radioactively labeled hormones, notably the β2-adrenergic receptor and its ligand, adrenaline. This receptor was later isolated from cell membranes, sequenced, and its structure elucidated by Kobilka using techniques almost solely used by chemists at the time1,2,3. They realized that the β2-adrenergic receptor had a very similar structure to rhodopsin, an ocular light-detecting receptor. Using the premise that other receptors with identical organization may therefore exist, Lefkowitz, Kobilka and others managed to identify more than a thousand GPCRs implicated in almost every physiological process from metabolism to cardiac rhythm and pain tolerance4. Their efforts have enabled a much deeper understanding of cellular biology and signal transduction, and GPCRs continue to have a major impact in the world of drug discovery, serving as the targets for countless pharmacological therapeutics.

Today, a great number of orphan GPCRs remain to be fully characterized. Enzo Life Sciences offers a wide range of products for GPCR characterization and screening, including calcium and cAMP detection kits, Screen-Well® compound libraries consisting of GPCR agonists and antagonists, as well as worry-free antibody sets for GPCRs and GPCR signaling (see table below).

 

Related Products

cAMP complete ELISA kit 

Most sensitive (0.037 pmol/ml) and complete colorimetric kit for intra- and extracellular cAMP quantification.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-163 96 wells 411.00 USD
Do you need bulk/larger quantities?
 

Direct cAMP ELISA kit 

Most sensitive (0.037 pmol/ml) colorimetric kit for intracellular cAMP quantification.
ELISA, Colorimetric detection | Print as PDF
 
ADI-900-066 96 wells 378.00 USD
Do you need bulk/larger quantities?
 

Cyclic Nucleotide Phosphodiesterase assay kit 

Unique colorimetric assay used to screen inhibitors and modulators of cyclic nucleotide phosphodiesterase activity.
Colorimetric detection, HTS, Activity assay | Print as PDF
 
BML-AK800-0001 96 wells 404.00 USD
Do you need bulk/larger quantities?
 

FLUOFORTE® Calcium assay kit  

A Brighter, More Robust Fluorescent Assay for Calcium Mobilization
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51017 10x96 tests 330.00 USD
Do you need bulk/larger quantities?
 

GFP-CERTIFIED® FLUOFORTE® Calcium assay kit 

A brighter, more robust and GFP-compatible (orange dye) fluorescent assay for calcium mobilization
Flow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS | Print as PDF
 
ENZ-51020-KP010 1 Kit 444.00 USD
 
ENZ-51020-KP100 1 Kit 3,188.00 USD
Do you need bulk/larger quantities?
 

SCREEN-WELL® ICCB Known Bioactives library 

HTS | Print as PDF
 
BML-2840-0100 1 Library 100 µl/well Inquire for pricing
Do you need bulk/larger quantities?
 

SCREEN-WELL® Neurotransmitter library (10 plate set) 

HTS | Print as PDF
 
BML-2810-0500 1 Library 500 µl/well Inquire for pricing
 
BML-2810-0100 1 Library 100 µl/well Inquire for pricing
Do you need bulk/larger quantities?
 

G Protein antibody set 

IHC, IP, WB | Print as PDF
 
BML-SA182-0001 1 Set 779.00 USD
Do you need bulk/larger quantities?
 

GPCR cardio, Ab sample pack 

ELISA, IHC, WB | Print as PDF
 
ADI-PAK-130 13x25 µg 799.00 USD
Do you need bulk/larger quantities?
 

GPCR obesity, Ab sample pack 

ELISA, IHC, WB | Print as PDF
 
ADI-PAK-120 6x25 µg 486.00 USD
Do you need bulk/larger quantities?
 

GPCR pain, Ab sample pack 

ELISA, IHC, WB | Print as PDF
 
ADI-PAK-110 5x25 µg 345.00 USD
Do you need bulk/larger quantities?
 

References:

Recommend this page

 
For Research Use Only. Not for use in diagnostic procedures.
Keep in touch

©2017 Enzo Life Sciences, Inc.,