Removes 5’-phosphates from DNA, RNA, dNTPs and proteins
Works in restriction enzyme buffers
Used in PCR clean-up to treat dNTPs prior to DNA sequencing or SNP analysis
Shrimp alkaline phosphatase (recombinant), (rSAP) is a multipurpose alkaline phosphatase that is fully inactivated by a short heat treatment. rSAP has all the properties of the native enzyme, but with the additional benefits of greater stability, consistency, and purity.
rSAP simplifies cloning and sequencing workflows due to a complete heat inactivation.
Product Details
Alternative Name:
rSAP
MW:
54 kDa
Source:
Pandalus borealis (Northern red shrimp) origin, recombinantly produced in Pichia pastoris.
UniProt ID:
Q9BHT8_PANBO
Concentration:
1U/µl for 1000 unit size
5U/µl for 5000 unit size
Formulation:
Liquid.
Purity Detail:
Purified by SDS-PAGE. DNase, RNase or protease activities not detected.
Specific Activity:
>2000 units/mg. One unit of Shrimp alkaline phosphatase (recombinant) converts 1µmol p-nitrophenyl phosphate per minute to nitrophenol and phosphate at 37°C and pH 10.4 in 0.1 M glycine buffer, 1 mM each of ZnCl2 and MgCl2 and 6 mM 4-nitrophenyl phosphate.
Applications:
Cloning
Shipping:
Blue Ice
Short Term Storage:
-20°C
Long Term Storage:
-20°C
Use/Stability:
Stable for 2 years at -20°C, for 6 months at 4°C, or for 3 months at 25°C
Regulatory Status:
RUO - Research Use Only
Fig 4. Sequencing workflow using Shrimp alkaline phosphatase (SAP)
Fig 3. Cloning workflow using Shrimp alkaline phosphatase (SAP)
Fig 2. Stability of Shrimp alkaline phosphatase at room temperature
Fig 1. Heat inactivation of Shrimp alkaline phosphatase at 65°C and 75°C
