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High-sensitivity ELISA enables quantification of low amounts of IL-6
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Easy-to-use liquid color-coded reagents reduce errors
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Rapid quantitative results in 3 hours
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High-throughput immunoassay measures up to 40 samples in duplicate per 96 well kit
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Ready-to-use reagents and pre-coated plates reduce errors and save time
The IL-6 (human), high sensitivity ELISA kit is a colorimetric immunoassay kit with results in 3 hours. Absorbance is read at 450 nm. Measure up to 40 samples in duplicate per 96 well kit.
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Product Details
| Alternative Name: | Interleukin-6 |
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| Sensitivity: | 0.057 pg/mL (1.56 - 50 pg/mL) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of human IL-6 in culture supernatants, plasma, serum, and urine. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human
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| Crossreactivity: | Negligible crossreactivity to IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-7, IL-8, TNFα, and TNFβ |
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| Use/Stability: | Store all components at +4°C, except standard at -20°C. |
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| Shipping: | Blue Ice |
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| Contents: | Microtiter plate, Conjugate, Antibody, Assay buffer 13, Wash buffer concentrate, Standard, TMB Substrate, Stop solution |
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| Scientific Background: | Interleukin-6 (IL-6) is a cytokine critical to the regulation of the immune and hematopoietic systems. Human IL-6 is expressed by T-cells, mast cells, monocytes, macrophages, fibroblasts, endothelial cells, keratinocytes, and many tumor cell lines. It plays a role in acute phase reactions and response to injury and inflammation. Human IL-6 also stimulates the differentiation of B-cells for antibody production, promotes expansion of activated T-cells, expands hematopoietic cell production, and induces the expression of acute phase proteins. |
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| UniProt ID: | P05231 |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Inflammatory and oxidative stress markers in intracerebral hemorrhage: Relevance as prognostic markers for quantification of the edema volume: V. Rendevski, et al.; Brain Pathol.
33, e13106 (2023),
Abstract;
Full Text
Resveratrol has its antioxidant and anti-inflammatory protective mechanisms decreased in aging: M.A. Santos, et al.; Arch. Gerontol. Geriatr.
107, 104895 (2023),
Abstract;
Anti-inflammatory properties of S53P4 bioactive glass implant material: F.N. Barrak, et al.; J. Dent.
127, 104296 (2022),
Abstract;
BACH1 is transcriptionally inhibited by TET1 in hepatocellular carcinoma in a microRNA-34a-dependent manner to regulate autophagy and inflammation: X. Sun, et al.; Pharmacol. Res.
169, 105611 (2021),
Abstract;
Infrared light therapy relieves TLR-4 dependent hyper-inflammation of the type induced by COVID-19: B. Aguida, et al.; Commun. Integr. Biol.
14, 200 (2021),
Application(s): IL-6 quantification on HEK293 cells supernatant,
Abstract;
Full Text
Sterile-filtered saliva is a strong inducer of IL-6 and IL-8 in oral fibroblasts: B. Cvikl, et al.; Clin. Oral. Investig.
19, 385 (2015),
Application(s): Cell culture Supernatant,
Abstract;
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