Highest sensitivity, complete staining kit for both IHC and ISH
POLYVIEW® PLUS reagents' biotin free nanopolymer detection circumvents endogenous background while providing specific antigen localization
HIGHDEF® DAB (Brown) HRP provides high intensity color development to deliver sharp, crisp staining
Compatible with both manual and automated platforms
The POLYVIEW® PLUS HRP-DAB (Anti-Rabbit) Kit, is intended for both IHC and ISH staining. The kit is a non-biotin one step detection system suitable for demonstrating antigens in formalin-fixed paraffin-embedded tissues and frozen sections. This kit may also be used with blood smears, cytosmears, and cell preparations.
POLYVIEW® PLUS HRP-DAB (Anti-Rabbit) Kit used to detect Ki-67 on cervical tissue.
POLYVIEW® PLUS HRP-DAB (Anti-Rabbit) Kit used to stain tonsil tissue.
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Product Details
Assay Time:
< 2 hours
Applications:
IHC ISH (in situ hybridization)
Handling:
Do not freeze.
Shipping:
Blue Ice Not Frozen
Long Term Storage:
+4°C
Contents:
IHC/ISH Peroxidase Block, Antibody Blocking Buffer, POLYVIEW® PLUS HRP (Anti-Rabbit) Reagent (Ready-To-Use), HIGHDEF® DAB Chromogen, HIGHDEF® DAB Substrate Buffer, HIGHDEF® Hematoxylin, Antigen Retrieval Reagent, pH 9 (10X)
Technical Info/Product Notes:
The POLYVIEW® PLUS HRP-DAB (Anti-Rabbit) Kit contains POLYVIEW® PLUS HRP (Anti-Rabbit) Reagent that has been developed by directly labeling anti-rabbit immunoglobulins with enzymes using a proprietary tandem hyper-labelling technology in ready-to-use format. This ensures consistent and reproducible immunodetection of rabbit antibodies against nuclear, cytoplasmic and membrane antigens in different types of tissues. The single step POLYVIEW® PLUS HRP (Anti-Rabbit) Reagent enables faster staining procedures compared to traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background. Enzo’s HIGHDEF® DAB (Brown) HRP and HIGHDEF® Hematoxylin reagents provide strong signal coupled with the peroxidase and antibody blockers included in the kit to give minimal background.
The POLYVIEW® PLUS HRP-DAB (Anti-Rabbit) Kit is suitable for use with all rabbit antibodies, both monoclonal and polyclonal. The reagent from this kit can be used for manual staining or with automated staining instruments and is well suited for multiplex immunohistochemical staining assays.
Regulatory Status:
RUO - Research Use Only
Product Literature References
Nanobody-based retargeting of an oncolytic herpesvirus for eliminating CXCR4 + GBM cells: A proof of principle: J.S. Gil, et al.; Mol. Ther. Oncolytics 26, 35 (2022), Abstract;
Afadin downregulation by Helicobacter pylori induces epithelial to mesenchymal transition in gastric cells: M.S. Marques, et al.; Front. Microbiol. 9, 2712 (2018), Abstract; Full Text
