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Sensitive measurement of methotrexate, detecting as little as 0.087 ng/ml
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Broad dynamic range suitable for a large variety of samples
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High throughput format with results in 1.5 hours for up to 38 samples in duplicate
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Fully quantitative results that surpass semi-quantitative Western blot analysis
The Methotrexate ELISA kit is a colorimetric competitive immunoassay kit with results in 1.5 hours.
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Product Details
| Alternative Name: | MTX |
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| Sensitivity: | 0.087 ng/ml (0.13-1000 ng/ml) |
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| Assay Time: | 1.5 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of methotrexate in serum, plasma and urine in human, mouse, and rat samples. |
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| Wavelength: | 450 nm |
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| Species reactivity: | Human, Mouse, Rat
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | Microtiter Plate, Assay Buffer 13, Standard, Wash Buffer Concentrate, Antibody, Antibody Diluent, Conjugate, Substrate, and Stop Solution |
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| Scientific Background: | Methotrexate is a drug used in the treatment of cancer and autoimmune disease. It is designed as an anti-folate to inhibit the metabolism of folic acid. Two distinct mechanisms of action have been described for methotrexate. In cancer treatments, methotrexate competitively inhibits the dihydrofolate reductase (DHFR) by blocking folate binding. DHFR converts dihydrofolate to active tetrahydrofolate. Inhibition of DHFR results in inhibition of the synthesis of purine and pyrimidine bases effectively limiting DNA and RNA synthesis and cancer cell growth. In autoimmune disease and specifically in the treatment of rheumatoid arthritis, methotrexate appears to impact several pathways resulting in inhibition of T cell activation. The effects include suppression of T cell expression of intercellular adhesion molecules, inhibition of methyl transferase activity and increased CD95 sensitivity leading to apoptosis in active T cells.
Monitoring methotrexate levels is important to assure appropriate levels are maintained during therapy or treatment. High levels of methotrexate can lead to toxicity and potential renal failure as well as immunosuppression. Additionally, methotrexate is known to interact with a wide variety of drugs leading to additional complications. Determining the presence of methotrexate in samples from subjects in blinded research studies can assist in the interpretation of study results.
Methotrexate is established as one of the most effective and safe therapeutics for rheumatoid arthritis. The safety profile assures that methotrexate will continue to be used in new studies in combination with other new or established drugs. The same is true in its use as a cancer therapeutic. The ELISA enables monitoring levels of methotrexate in both preclinical and clinical research. The methotrexate assay is also appropriate for the detection of methotrexate contamination after its use as a selective agent for recombinant protein production in mammalian cell lines. |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader.
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Product Literature References
Choroid plexus-CSF-targeted antioxidant therapy protects the brain from toxicity of cancer chemotherapy: A. Jang, et al.; Neuron
110, 3288 (2022),
Abstract;
Discovery of a Highly Specific Anti-methotrexate (MTX) DNA Aptamer for Antibody-Independent MTX Detection: M. Nagano, et al.; Anal. Chem.
94, 17255 (2022),
Abstract;
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