Antifade formulation inhibits rapid photobleaching of fluorescent proteins and fluorescent dyes
No added counterstain allows for custom titration of DAPI as desired
Ready-to-use non-hardening formulation
Refractive index: 1.45
SEEBRIGHT® Mounting Medium is an antifade reagent that minimizes photobleaching. Use of this mounting medium significantly extends the lifetime of fluorescent signals.
Product Details
Quantity:
Each vial of SEEBRIGHT® Mounting Medium contains 10 mL sufficient for 1000 tests (ENZ-GEN-420-1000). Apply mounting medium to the sample area as desired.
Applications:
IF, Fluorescence microscopy, Fluorescent detection FISH
Shipping:
Blue Ice
Short Term Storage:
+4°C
Long Term Storage:
+4°C
Handling:
Protect from light. Do not freeze.
Scientific Background:
All fluorescent dyes signal fade (photobleach) when exposed to excitation light. The amount of photobleaching depends on the composition of the molecule and the time of light exposure. SEEBRIGHT® Mounting Medium can be used to prevent photobleaching and prolong fluorescent signals. SEEBRIGHT® Mounting Medium is compatible with many dyes, including Enzo's SEEBRIGHT® Fluorescently-Lableled dUTPs.
Technical Info/Product Notes:
SEEBRIGHT® Mounting Medium is recommended for use on fixed cells and tissues that have been stained with fluorescent dyes or hybridized with fluorescent probes. Cells can be viewed immediately or mounted slides can be stored in the dark at 4°C. SEEBRIGHT® Mounting Medium is a non-hardening formula. Hence, slides with coverslips can be stored unsealed or coverslips can be sealed around their perimeter using nail polish or a plastic sealant.
Regulatory Status:
RUO - Research Use Only
General Literature References
Influence of refractive-index mismatch in high-resolution three-dimensional confocal microscopy.: A. Diaspro et al.; Appl. Opt. 41, 685 (2002), Abstract;
Quantitative comparison of anti-fading mounting media for confocal laser scanning microscopy. : M. Ono et al.; J. Histochem. Cytochem. 49, 305 (2001), Abstract; Full Text
