Unique antifade formulation inhibits rapid photobleaching of fluorescent proteins and fluorescent dyes
Ready-to-use non-hardening formulation
Includes DAPI counterstain at 1.5 µg/ml
Refractive index: 1.45
SEEBRIGHT® Mounting Medium with DAPI is an antifade reagent that minimizes photobleaching. Use of the mounting medium significantly extends the lifetime of fluorescent signals. The inclusion of DAPI allows for staining of the nucleus.
Product Details
Quantity:
Each vial of SEEBRIGHT® Mounting Medium with DAPI contains 200 µL sufficient for 20 tests (ENZ-GEN420-0020) or 1000 µL sufficient for 100 tests (ENZ-GEN420-0100). Apply mounting medium to the sample area as desired.
Applications:
IF, Fluorescence microscopy, Fluorescent detection FISH
Shipping:
Blue Ice
Short Term Storage:
+4°C
Long Term Storage:
+4°C
Handling:
Protect from light. Do not freeze.
Scientific Background:
All fluorescent dyes fade (photobleach) when exposed to excitation light. The amount of photobleaching depends on the composition of the molecule and the time of light exposure. SEEBRIGHT® Mounting Medium with DAPI can be used to prevent photobleaching and prolong fluorescent signals. This formulation contains 4′,6-diamidino-2-phenylindole (DAPI), which serves as a nuclear or chromosomal counterstain that produces a blue fluorescence when bound to DNA. SEEBRIGHT® Mounting Medium with DAPI is compatible with many dyes, including Enzo's SEEBRIGHT® Fluorescently-Labeled dUTPs.
Technical Info/Product Notes:
SEEBRIGHT® Mounting Medium with DAPI is recommended for use on fixed cells and tissues that have been stained with fluorescent dyes or hybridized with fluorescent probes. Cells can be viewed immediately, or stored in the dark at 4°C. SEEBRIGHT® Mounting Medium with DAPI is a non-hardening formula. Hence, slides with coverslips can be stored unsealed or coverslips can be sealed around their perimeter using nail polish or a plastic sealant. DAPI excites at about 360 nm and emits at about 460 nm. DAPI is a mutagen and appropiate precautions should be taken.
Regulatory Status:
RUO - Research Use Only
General Literature References
Influence of refractive-index mismatch in high-resolution three-dimensional confocal microscopy.: A. Diaspro et al.; Appl. Opt. 41, 685 (2002), Abstract;
Quantitative comparison of anti-fading mounting media for confocal laser scanning microscopy. : M. Ono et al.; J. Histochem. Cytochem. 49, 305 (2001), Abstract; Full Text
