Human Genomic DNA, Male, is comprised of a pool of ≥ 8 normal donors. It is intact DNA extracted from freshly harvested whole blood of healthy males. DNA is treated with DNase free-RNase to remove residual contaminant RNA.
Human genomic DNA can be used for a variety of molecular applications including PCR, southern blotting, and DNA library construction. It can also be used as normal reference DNA for comparative genomic hybridizations (CGH).
Product Details
Alternative Name:
gDNA
Concentration:
150 - 250 µg/mL in 10 mM Tris-HCl, pH 8.0, containing 1 mM EDTA
Formulation:
Liquid.
Quality Control:
A260/280 is between 1.8 and 2.0
Sex specificity determined by PCR
The integrity of each DNA sample is verified by agarose gel electrophoresis before and after incubation at 37°C for 16 hours
Genomic DNA is successfully digested by AluI and RsaI and migration on agarose gel was as expected
Molecular weight is greater than 20 kb
Applications:
PCR, Southern Blot, CGH
Application Notes:
Genomic DNA is suitable for PCR, Southern blot analysis, DNA library construction, and used as reference DNA in CGH and other hybridizations.
Shipping:
Blue Ice
Short Term Storage:
+4°C
Long Term Storage:
-20°C
Handling:
Avoid freeze/thaw cycles.
Regulatory Status:
RUO - Research Use Only
Product Literature References
An innovative transfer DNA experimental design and qPCR assay: Protocol and pilot study: S.M. McCrane, et al.; J. Forensic Sci. 68, 990 (2023), Abstract;
