Product Details
| Alternative Name: | HMWCK, High molecular weight cytokeratin, Keratin-903 |
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| Clone: | 34βE12 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Keratin solubilized from human stratum corneum. |
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| UniProt ID: | P04264 (CK1), P13647 (CK5), P13645 (CK10), P02533 (CK14) |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human
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| Specificity: | 34βE12 is specific for "high molecular weight" cytokeratins 1, 5, 10 and 14, corresponding to molecular weights 68, 58, 56.5 and 50 kDa, respectively, which are characteristically found in "complex" epithelium. The antibody reacts with all squamous and ductal epithelium as well as carcinomas such as squamous cell carcinoma and adenocarcinoma of ductal origin (e.g., breast, pancreas) but does not react with sarcoma, melanoma or lymphoma. This antibody reacts with benign small-acinar lesions of the prostate and has been shown to be useful as an adjunct in the identification of prostate carcinoma. |
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| Applications: | IHC (PS), WB
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| Recommended Dilutions/Conditions: | Immunohistochemistry (paraffin sections; For optimal results, fixation in alcohol-based fixatives, such as methacarn, is recommended. Pretreatment with proteolytic enzymes such as trypsin may be required when formalin-fixed sections are to be stained. For optimal staining of prostate tissue higher concentrations of protease and longer incubation times are recommended, e.g., 0.5% trypsin at 37°C for 2 to 4 hours. Choose a good quality enzyme-linked anti-mouse IgG1 antibody for detection).
General suggested starting dilution is 1:20-1:50.
Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each appliation. |
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| Purity Detail: | Affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.1% sodium azide and 1% BSA. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of human skin tissue using Cytokeratin 1/5/10/14 (human), mAb (34βE12), (Prod. No. ENZ-C34903) at a dilution of 1:20.
Immunohistochemistry analysis of human skin tissue using Cytokeratin 1/5/10/14 (human), mAb (34βE12), (Prod. No. ENZ-C34903) at a dilution of 1:20.
Immunohistochemistry analysis of human skin tissue using Cytokeratin 1/5/10/14 (human), mAb (34βE12), (Prod. No. ENZ-C34903) at a dilution of 1:20.
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Product Literature References
Molecular evidence that invasive adenocarcinoma can mimic prostatic intraepithelial neoplasia (PIN) and intraductal carcinoma through retrograde glandular colonization: M.C. Haffner, et al.; J. Pathol.
238, 31 (2016),
Abstract;
Full Text
Unusual manifestations of secondary urothelial carcinoma: C.L. Zhao, et al.; Human Pathology: Case Reports 3, 1 (2016), Application(s): Immunohistochemical antibodies used for diagnosis of cases,
Metastasis of Malignant Melanoma to Urinary Bladder: A Case Report and Review of the Literature: R. Meunier, et al.; Case Rep. Pathol.
2015, 173870 (2015),
Application(s): Immunohistochemistry,
Abstract;
Full Text
Cytoplasmic PTEN protein loss distinguishes intraductal carcinoma of the prostate from high-grade prostatic intraepithelial neoplasia: T.L. Lotan, et al.; Mod. Pathol.
26, 587 (2013),
Abstract;
Full Text
Immunohistochemical markers for the differential diagnosis of nephrogenic adenomas: W. Quinones, et al.; Ann. Diagn. Pathol.
17, 41 (2013),
Abstract;
Immunohistochemical Staining with DeltaNp63 Is Useful for Distinguishing the Squamous Cell Component of Adenosquamous Cell Carcinoma of the Lung: H. Uramoto, et al.; Anticancer Res.
30, 4717 (2010),
Application(s): IHC using human lung cancer (ADSQ) samples,
Abstract;
Full Text
Somatic but Not Germline Mutation of the APC Gene in a Case of Cribriform-Morular Variant of Papillary Thyroid Carcinoma: J. Cameselle-Teijeiro, et al.; Am. J. Clin. Pathol.
115, 486 (2001),
Application(s): IHC using human thyroid carcinoma samples,
Abstract;
Full Text
Androgen responsive adult human prostatic epithelial cell lines immortalized by human papillomavirus 18: D. Bello, et al.; Carcinogenesis
18, 1215 (1997),
Application(s): ICC using human prostate cell lines RWPE-1 and -2,
Abstract;
Full Text
Metaplastic Shadow Cells in Rectal Adenocarcinoma: Report of a Case With Immunohistochemical Study: H. Nakayama, et al.; Jpn. J. Clin. Oncol.
27, 427 (1997),
Application(s): IHC using human rectal adenocarcinoma,
Abstract;
Full Text
Use of keratin 903 as an adjunct in the diagnosis of prostate carcinoma: L. Hedrick & J.I. Epstein; Am. J. Surg. Pathol.
13, 389 (1989),
Abstract;
Basal cell hyperplasia, adenoid basal cell tumor, and adenoid cystic carcinoma of the prostate gland: an immunohistochemical study: D.J. Grigon, et al.; Hum. Pathol.
19, 1425 (1988),
Abstract;
Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues: A.M. Gown & A.M. Vogel; Am. J. Pathol.
114, 309 (1984),
Abstract;
Full Text
Monoclonal antibodies to intermediate filament proteins of human cells: unique and cross-reacting antibodies: A.M. Gown & A.M. Vogel; J. Cell Biol.
95, 414 (1982),
Abstract;
Full Text
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