High intensity color development delivers sharp, crisp staining
Compatible with both automated and manual platforms
The Antibody Blocker/Diluent reduces background further (sold separately)
Customize with HIGHDEF® chromogens of your choice (sold separately)
The POLYVIEW® PLUS AP (anti-rabbit) reagent is a ready-to-use, non-biotin, one-step detection reagent suitable for immunohistochemical detection of antigens in formalin-fixed paraffin-embedded tissues and frozen sections. The detection reagent may also be used with blood smears, cytosmears, and cell preparations. In addition, the POLYVIEW® PLUS AP (anti-rabbit) reagent can be adapted for in situ hybridization detection.
Product Details
Applications:
IHC ISH (in situ hybridization)
Quantity:
150 tests × 150 µL (22.5 mL total volume)
Handling:
Do not freeze.
Shipping:
Blue Ice Not Frozen
Long Term Storage:
+4°C
Technical Info/Product Notes:
The POLYVIEW® PLUS AP (anti-rabbit) reagent has been developed by directly labeling anti-rabbit immunoglobulins with a nanopolymer of alkaline phosphatase (AP) using proprietary technology. This ensures consistent and reproducible immunodetection of rabbit antibodies against nuclear, cytoplasmic, and membrane antigens in different types of tissues. The single step methodology enables faster staining procedures than traditional two-step methods using biotin and avidin/streptavidin conjugates, with significantly lower background.
The POLYVIEW® PLUS AP (anti-rabbit) reagent is suitable for use with rabbit IgG antibodies, both monoclonal and polyclonal. The reagent can be used for manual staining or with automated staining instruments and are well suited for multiplex immunohistochemical staining assays.
The Antibody Blocker/Diluent (ENZ-ACC108) further reduces background and is suitable for diluting the POLYVIEW® PLUS AP (anti-rabbit) reagent.
Regulatory Status:
RUO - Research Use Only
Tonsil tissue stained with anti-Ki-67 antibody (rabbit), followed by POLYVIEW® PLUS AP (anti-rabbit) reagent
Dual IHC staining performed on formalin-fixed paraffin-embedded tissue section from human brain with Alzheimer’s disease. The tissue sections were stained for alpha-amyloid with POLYVIEW® PLUS AP (anti-rabbit) reagent (Prod. No. ENZ-ACC110) and HIGHDEF® Green AP Chromogen/Substrate (Prod. No. ENZ-ACC130), and phospho-tau with POLYVIEW® PLUS HRP (anti-mouse) reagent (Prod. No. ENZ-ACC104) and HIGHDEF DAB Chromogen/Substrate Set (Prod. No. ENZ-ACC105). Picture courtesy of Dr. Karelle Leroy Laboratoire d’histologie, de neuroanatomie et de neuropathologie, Université Libre de Bruxelles, Bruxelles, Belgium.
Dual IHC staining performed on formalin-fixed paraffin-embedded tissue section from transgenic mouse brain expressing human mutated APP and PS1. The tissue sections were stained for alpha-amyloid with POLYVIEW® PLUS AP (anti-rabbit) reagent (Prod. No. ENZ-ACC110) and HIGHDEF® Green AP Chromogen/Substrate (Prod. No. ENZ-ACC130), and phospho-tau with POLYVIEW® PLUS HRP (anti-mouse) reagent (Prod. No. ENZ-ACC104) and HIGHDEF DAB Chromogen/Substrate Set (Prod. No. ENZ-ACC105). Picture courtesy of Dr. Karelle Leroy Laboratoire d’histologie, de neuroanatomie et de neuropathologie, Université Libre de Bruxelles, Bruxelles, Belgium.
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Product Literature References
Clinical course and challenging management of early COVID-19 infection after heart transplantation: case report of two patients: V. Tchana-Sato, et al.; BMC Infect. Dis. 21, 89 (2021), Abstract; Full Text
