Purified from hybridoma tissue culture supernatant.
Species reactivity:
Human, Mouse, Rat Bovine, Chicken
Applications:
ICC, IF, IHC (PS), WB
Recommended Dilutions/Conditions:
Immunohistochemistry (paraffin sections, 10µg/ml) Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.
Application Notes:
Detects a band of ~20kDa by Western blot.
Purity Detail:
Protein G affinity purified.
Formulation:
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Handling:
Avoid freeze/thaw cycles.
Shipping:
Blue Ice
Long Term Storage:
-20°C
Scientific Background:
Alpha-crystallins, which are part of the small Heat shock family members, are major water-soluble proteins present in the lens of the mammalian eye. Phosphorylation of serine residues which occurs during development and in response to stress, is intimately linked with its function. Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation.
Regulatory Status:
RUO - Research Use Only
Western Blot analysis of αB-Crystallin mAb (1B6.1-3G4) (Prod. No. ENZ-ABS706): Lane 1: MW Marker, Lane 2: α-Crystallin (bovine), (native) (Prod. No. ADI-SPP-225), Lane 3: αB-Crystallin (bovine), (native) (Prod. No. ADI-SPP-227).
Immunohistochemistry analysis of human heart tissue with alpha B-Crystallin, mAb (1B6.1-3G4), (Prod. No. ENZ-ABS706), at 20µg/ml.
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Product Literature References
The small heat shock protein αB-Crystallin protects versus withaferin A-induced apoptosis and confers a more metastatic phenotype in cisplatin-resistant ovarian cancer cells: M.M. Carmichael, et al.; PLoS One 18, e0281009 (2023), Abstract;