The GFP-CERTIFIED® FLUOFORTE® Reagent (Non-AM) is the most sensitive fluorescent calcium indicator relative to other commercially available red fluorescent dyes. It can be used for detecting intracellular calcium mobilization across a broad spectrum of biological targets. Esterase hydrolysis is not required for live cell analysis via flow cytometry or fluorescence microscopy.
Product Details
MW:
~800
Excitation maximum:
524nm
Emission maximum:
551nm
Purity:
≥95% (HPLC)
Appearance:
Lyophilized.
Solubility:
Soluble in water.
Shipping:
Dry Ice
Short Term Storage:
-20°C
Long Term Storage:
-80°C
Handling:
Protect from moisture. Protect from light. Avoid freeze/thaw cycles.
Technical Info/Product Notes:
The GFP-CERTIFIED® FLUOFORTE® Reagent is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding cell analysis applications involving confocal microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required.
Based on application requirement, we also offer ENZ-52016.
Regulatory Status:
RUO - Research Use Only
General Literature References
Novel fluo-4 analogs for fluorescent calcium measurements: V.V. Martin, et al.; Cell Calcium 36, 509 (2004), Abstract;
Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41: M. do Ceu Monteiro, et al.; Cytometry 35, 302 (1999), Abstract;