5(6)-CDCFDA is an amine–reactive fluorescein diacetate (FDA) derivative used to prepare a variety of FDA conjugates. 5(6)-CDCFDA is a non-fluorescent molecule that diffuse into cells and is hydrolyzed by intracellular non-specific esterases, yielding a fluorescent probe. The probe accumulates exclusively in cells with intact cell membranes. 5(6)-CFCFDA does not stain dead cells. 5(6)-CDCFDA labeling can be used for monitoring cells by flow cytometry or fluorescence microscopy. The probe is also frequently used to monitor reactive oxygen species (ROS) generation in live cells. Wavelength Maxima: Excitation 504nm, Emission 529nm
Product Details
| Alternative Name: | 5(6)-Carboxy-2’,7’-dichlorofluorescein diacetate |
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| Formula: | C25H14Cl2O9 |
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| MW: | 529.3 |
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| CAS: | 127770-45-0 |
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| Purity: | ≥98% (HPLC) |
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| Solubility: | Soluble in DMSO. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Application(s): ROS detection in human endothelial cells,
Abstract;
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