The GFP-CERTIFIED® FLUOFORTE® Reagent is the brightest and most sensitive fluorescent calcium indictor relative to other commercially available red fluorescent dyes with maximum excitation at ~530nm and maximum emission at ~555nm. This product is used in a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.
Protect from moisture. Protect from light. Avoid freeze/thaw cycles.
Technical Info/Product Notes:
The GFP-CERTIFIED® FLUOFORTE® Reagent is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding cell analysis applications involving confocal microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required.
Regulatory Status:
RUO - Research Use Only
Product Literature References
Sulfur Dioxide Protects Against Collagen Accumulation in Pulmonary Artery in Association With Downregulation of the Transforming Growth Factor β1/Smad Pathway in Pulmonary Hypertensive Rats: W. Yu, et al.; J. Am. Heart Assoc. 5, e003910 (2016), Application(s): Detection of calcium levels in PAFs and PASMCs, Abstract; Full Text
GABAergic signaling in the pulmonary neuroepithelial body microenvironment: functional imaging in GAD67-GFP mice: K. Schnorbusch, et al.; Histochem. Cell. Biol. 140, 549 (2013), Abstract;
FLIM FRET technology for drug discovery: automated multiwell-plate high-content analysis, multiplexed readouts and application in situ: S. Kumar, et al.; Chemphyschem 12, 609 (2011), Application(s): Calcium levels in HEK293 cells, Abstract; Full Text
General Literature References
Novel fluo-4 analogs for fluorescent calcium measurements: V.V. Martin, et al.; Cell Calcium 36, 509 (2004), Abstract;
Calcium measurements in perfused mouse heart: quantitating fluorescence and absorbance of Rhod-2 by application of photon migration theory: C. Du, et al.; Biophys J. 80, 549 (2001), Full Text
Rhod-2 based measurements of intracellular calcium in the perfused mouse heart: cellular andsubcellular localization and response to positive inotropy: G.A. MacGowan, et al. ; J. Biomed. Opt. 6, 23 (2001), Abstract;
Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41: M. do Ceu Monteiro, et al.; Cytometry 35, 302 (1999), Abstract;
Nucleoplasmic and cytoplasmic differences in the fluorescence properties of the calcium indicator Fluo-3: C. Perez-Terzic, et al.; Cell Calcium 21, 275 (1997), Abstract;
Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets.: R. Greimers, et al. ; Cytometry 23, 205 (1996), Abstract;
