Replaces Prod. #: ALX-620-004
Fura-2 is one of the most widely used calcium indicators for homogenous quantitative dual wavelength ratiometric cell measurements. Fura-2 is particularly useful for digital imaging microscopy. It is less susceptible to photobleaching than Indo-1. The probe is excited only by UV light, which results in significantly less interference by visible wavelength excitable fluorescent compounds. One application of the probe is as a useful counter screen tool for GPCR and calcium channels identified using calcium indicators excited at 488nm in primary screens. Fura-2 is a membrane impermeable probe and can be loaded in cells by microinjection, electroporation or scrape loading. The acetomethoxy (AM) version is necessary for passive cell loading. Wavelength Maxima: Excitation 363nm, Emission 512nm
Product Details
| Alternative Name: | 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxy]-2-(2'-amino-5'-methyl-phenoxy)ethane-N,N,N',N'-tetraacetic acid . 5K |
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| Formula: | C29H22K5N3O14 |
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| MW: | 832.0 |
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| CAS: | 113694-64-7 |
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| Purity: | ≥95% (HPLC) |
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| Appearance: | Light yellow solid. |
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| Solubility: | Soluble in water. |
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| Shipping: | Ambient Temperature |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least one year after receipt when stored as recommended. |
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| Handling: | Protect from light. Keep cool and dry. |
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| Technical Info/Product Notes: | This product is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding imaging applications, such as confocal microscopy, flow cytometry and HCS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Genome-wide CRISPR-Cas9 screens identify mechanisms of BET bromodomain inhibitor sensitivity: D. Estoppey, et al.; iScience
24, 103323 (2021),
Abstract;
Novel high-throughput assay to assess cellular manganese levels in a striatal cell line model of Huntington's disease confirms a deficit in manganese accumulation: G.F. Kwakye, et al.; Neurotoxicology
32, 630 (2011),
Abstract;
Full Text
Measurement of [Ca2+]i in whole cell suspensions using fura-2: R.A. Hirst, et al.; Methods Mol. Biol.
312, 37 (2006),
Abstract;
