- Specifically designed for use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFPs, CFPs, YFPs)
- Suitable for use with live or post-fixed cells
- Highly resistant to photo-bleaching, concentration quenching and photoconversion
- Suitable for use in conjunction with fluorescein- or coumarin-labeled antibodies
Enzo Life Sciences ER-ID® Red assay kit (GFP-CERTIFIED®) contains a red-emitting, cell-permeable small molecule organic probe to stain Endoplasmic Reticulum (ER) in live cell, or fixed cells. The dye emits in the Texas Red region of the visible light spectrum and can be readily used in combination with other common UV and visible light excitable organic fluorescent dyes/fluorescent proteins in multi-color imaging and detection applications. The kit includes also the Hoechst 33342 dye for the nuclear staining.
Figure 1: Live HeLa cells stained with ER-ID® Red dye (A), Hoechst dye (B) and resulting composite image (C).
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Product Details
| Alternative Name: | Endoplasmic reticulum / Organelle |
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| Applications: | Fluorescence microscopy
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| Application Notes: | Enzo Life Sciences’ ER-ID® Red assay kit (GFP-CERTIFIED®) contains a novel endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining. |
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| Quality Control: | A sample from each lot of ER-ID® Red assay kit is used to stain HeLa cells, using the procedures described in the user manual. The selectivity of the ER-ID® Red dye is evident. |
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| Quantity: | 500 assays |
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| Use/Stability: | With proper storage, the kit components are stable up to the date noted on the product label. Store kit at -20°C in a non-frost free freezer, or -80°C for longer term storage. |
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| Handling: | Protect from light. Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | -20°C |
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| Long Term Storage: | -80°C |
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| Contents: | ER-ID® Red detection reagent: 50μl
Hoechst 33342 nuclear stain: 50μl
10X assay buffer: 15ml |
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| Technical Info/Product Notes: | The ER-ID® Red assay kit (GFP-CERTIFIED®) is a member of the CELLESTIAL® product line, reagents and assay kits comprising fluorescent molecular probes that have been extensively benchmarked for live cell analysis applications. CELLESTIAL® reagents and kits are optimal for use in demanding cell analysis applications involving confocal microscopy, flow cytometry, microplate readers and HCS/HTS, where consistency and reproducibility are required. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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Secretion of a low‐molecular‐weight species of endogenous GRP94 devoid of the KDEL motif during endoplasmic reticulum stress in Chinese hamster ovary cells: A. Samy, et al.; Traffic
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Decoy receptor 3 promotes preosteoclast cell death via reactive oxygen species-induced fas ligand expression and the IL-1α/IL-1 receptor antagonist pathway: Y.J. Peng, et al.; Mediators. Inflamm.
2020, 1237281 (2020),
Application(s): Fluorescence microscopy,
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Differential effects of reactive oxygen species on IgG versus IgM levels in TLR-stimulated B cells: K.M. Gilljam, et al.; J. Immunol.
204, 2133 (2020),
Application(s): Flow cytometry,
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Effect of 4-phenylbutyrate and valproate on dominant mutations of WFS1 gene in Wolfram syndrome: K. Batjargal, et al.; J. Endocrinol. Invest.
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TRPV1 antagonist DWP05195 induces ER stress-dependent apoptosis through the ROS-p38-CHOP pathway in human ovarian cancer cells: Y.Y. Wang, et al.; Cancers (Basel)
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Application(s): Flow cytometry and fluorescence microscopy,
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Analysis of intracellular IgG secretion in Chinese hamster ovary cells to improve IgG production: K. Kaneyoshi, et al.; J. Biosci. Bioeng.
127, 107 (2019),
Application(s): Fluorescence microscopy,
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Application of Imaging Flow Cytometry for the Characterization of Intracellular Attributes in Chinese Hamster Ovary Cell Lines at the Single-Cell Level: E. Pekle, et al.; Biotechnol. J.
14, e1800675 (2019),
Application(s): High-throughput analysis of CHO cells with flow cytometry,
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CXCR2 specific endocytosis of immunomodulatory peptide LL-37 in human monocytes and formation of LL-37 positive large vesicles in differentiated: Z. Zhang, et al.; Bone Rep.
12, 100237 (2019),
Application(s): Fluorescence microscopy,
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Inhibition of eIF2α dephosphorylation accelerates pterostilbene-induced cell death in human hepatocellular carcinoma cells in an ER stress and autophagy-dependent manner: C.L. Yu, et al.; Cell Death Dis.
10, 418 (2019),
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Secretion analysis of intracellular “difficult-to-express” immunoglobulin G (IgG) in Chinese hamster ovary (CHO) cells: K. Kaneyoshi, et al.; Cytotechnology
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Variations of Intracellular CaMobilization Initiated by Nanosecond and Microsecond Electrical Pulses in HeLa Cells: N. Ohnishi, et al.; IEEE Trans. Biomed. Eng.
66, 2259 (2019),
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A pharmacochaperone-based high-throughput screening assay for the discovery of chemical probes of orphan receptors: C.J. Morfa, et al.; Assay Drug Dev. Technol.
16, 384 (2018),
Application(s): Fluorescence microscopy,
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Full Text
Induction of endoplasmic reticulum stress and mitochondrial dysfunction dependent apoptosis signaling pathway in human renal cancer cells by norcantharidin: M.H. Wu, et al.; Oncotarget
9, 4787 (2018),
Application(s): Fluorescence microscopy,
Abstract;
Full Text
Protodioscin induces apoptosis through ROS-mediated endoplasmic reticulum stress via the JNK/p38 activation pathways in human cervical cancer cells: C.L. Lin, et al.; Cell. Physiol. Biochem.
46, 322 (2018),
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Domain architecture of vasohibins required for their chaperone-dependent unconventional extracellular release: T. Kadonosono, et al.; Protein Sci.
26, 452 (2017),
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AT-101 simultaneously triggers apoptosis and a cytoprotective type of autophagy irrespective of expression levels and the subcellular localisation of Bcl-xL and Bcl-2 in MCF7 cells : P. Antonietti, et al. ; Biochim. Biophys. Acta
1863, 499 (2016),
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Abstract;
Biogenesis of the crystalloid organelle in Plasmodium involves microtubule-dependent vesicle transport and assembly: S. Saeed, et al.; Int. J. Parasitol.
45, 537 (2015),
Application(s): Microscopy,
Abstract;
Synthesis of a peptide that can translocate to the endoplasmic reticulum: K. Nakatsu, et al.; Biochem. Biophys. Res. Commun.
460, 328 (2015),
Application(s): Fluorescent Assay,
Abstract;
Target identification of grape seed extract in colorectal cancer using drug affinity responsive target stability (DARTS) technique: role of endoplasmic reticulum stress response proteins: M.M. Derry, et al.; Curr. Cancer Drug Targets
14, 323 (2014),
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Targeted protein destabilization reveals an estrogen-mediated ER stress response: K. Raina, et al.; Nat. Chem. Biol.
10, 957 (2014),
Application(s): Confocal microscopy,
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Full Text
Enhanced optineurin E50K-TBK1 interaction evokes protein insolubility and initiates familial primary open-angle glaucoma: Y. Minegishi, et al.; Hum. Mol. Genet.
22, 3559 (2013),
Abstract;
Knockdown of RyR3 enhances adiponectin expression through an atf3-dependent pathway: S.H. Tsai, et al.; Endocrinology
154, 1117 (2013),
Abstract;
Molecular cloning and tumour suppressor function analysis of canine REIC/Dkk-3 in mammary gland tumors: K. Ochiai, et al.; Vet. J.
197, 769 (2013),
Application(s): ER staining in dog mammary carcinoma cells,
Abstract;
Snowflake vitreoretinal degeneration (SVD) mutation R162W provides new insights into Kir7.1 ion channel structure and function: B.R. Pattnaik, et al.; PLoS One
8, e71744 (2013),
Application(s): ER staining in CHO-K1 cells,
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Full Text
General Literature References
Photoconversion of Lysotracker Red to a green fluorescent molecule: E.C. Freundt, et al.; Cell Res.
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Abstract;
Systematic colocalization errors between acridine orange and EGFP in astrocyte vesicular organelles: F. Nadrigny, et al.; Biophys. J.
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Chloromethyl-X-rosamine (MitoTracker Red) photosensitises mitochondria and induces apoptosis in intact human cells: T. Minamikawa, et al.; J. Cell Sci.
112, 2419 (1999),
Abstract;
Chloromethyltetramethylrosamine (Mitotracker Orange) induces the mitochondrial permeability transition and inhibits respiratory complex I. Implications for the mechanism of cytochrome c release: L. Scorrano, et al.; J. Biol. Chem.
274, 24657 (1999),
Abstract;
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