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NGAL (rat) ELISA kit

 
BPD-KIT-046 96 wells 906.00 USD
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  • Ready-to-use - pre-coated plate and individual calibrators save time and reduce errors
  • Fast - results from up to 40 samples in duplicate in just 4 hours per 96 well plate
A colorimetric immunometric ELISA kit for the rapid measurement of NGAL in rat samples. Absorbance is read at 450nm.
NGAL (rat) ELISA kit Standard curve
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NGAL (rat) ELISA kit Standard curve

Product Details

Alternative Name:Neutrophil gelatinase-associated lipocalin, Lipocalin 2, Siderocalin, Oncogene 24p3
 
Sensitivity:0.5pg/ml (range 4-400pg/ml)
 
Assay Time:4 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of NGAL in rat urine, plasma, serum, tissue extracts, or culture supernatants.
 
Wavelength:450 nm
 
Species reactivity:Rat
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Contents:1: Microwell plate coated with anti-NGAL (rat) antibody (96 wells) 2: 5X Sample Diluent 3: NGAL Rat Calibrators 1-8. 4: 25X Wash Solution Concentrate 5: Biotinylated Rat NGAL Antibody 6: HRP-Streptavidin 7: TMB Substrate 8: Stop Solution
 
Scientific Background:NGAL (also known as Lipocalin-2 or Neutrophil Gelatinase-associated Lipocalin) is a small, ~25kDa secreted protein expressed in epithelial tissues throughout the body.
 
Technical Info/Product Notes:For the manual please click here.
 
UniProt ID:P30152
 
Regulatory Status:RUO - Research Use Only
 
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

Plate Reader Just Got Personal
 
Manufacturer:Manufactured by BioPorto Diagnostics.
 

Product Literature References

Differences in the association between behavior and neutrophil gelatinase-associated lipocalin in male and female rats after coronary artery ligation: L. Gouweleeuw, et al.; Physiol. Behav. 163, 7 (2016), Abstract;
Effects of obesity and sex on antimicrobial pharmacokinetics and acute kidney injury: validation of a preclinical model: M.P. Pai, et al.; Antimicrob. Agents Chemother. 57, 716 (2013), Abstract;
Phosphodiesterase-5 inhibition attenuates early renal ischemia-reperfusion-induced acute kidney injury: assessment by quantitative measurement of urinary NGAL and KIM-1: R. Sohotnik, et al.; Am. J. Physiol. Renal Physiol. 304, F1099 (2013), Abstract;
Serum Lipocalin2 is a potential biomarker of liver irradiation damage: S. Sultan, et al.; Liver Int. 33, 459 (2013), Abstract;
Angiotensin-(1-7) attenuates diabetic nephropathy in Zucker diabetic fatty rats: J.F. Giani, et al.; Am. J. Physiol. Renal Physiol. 302, F1606 (2012), Abstract;
LIPOCALIN-2 is a major acute-phase protein in a rat and mouse model of sterile abscess: S. Sultan, et al.; Shock 37, 191 (2012), Abstract;
Role of peroxynitrite in sepsis-induced acute kidney injury in an experimental model of sepsis in rats: M. Seija, et al.; Shock 38, 403 (2012), Abstract;
Treatment with the vascular disrupting agent combretastatin is associated with impaired AQP2 trafficking and increased urine output: A.B. Bohn, et al.; Am. J. Physiol. Regul. Integr. Comp. Physiol. 303, R186 (2012), Abstract;
Urinary aminopeptidase activities as early and predictive biomarkers of renal dysfunction in cisplatin-treated rats: A. Quesada, et al.; PLoS One 7, e40402 (2012), Abstract;
Hypothermic renal perfusion during aortic surgery reduces the presence of lipocalin-2 and preserves renal extraction of dimethylarginines in rats: K.K. Yeung, et al.; Am. J. Physiol. Renal Physiol. 301, F1231 (2011), Abstract;
Inhibition of IОєB Kinase ОІ attenuates hypoxia-induced inflammatory mediators in rat renal tubular cells: X. Wan, et al.; Transplant Proc. 43, 1503 (2011), Abstract;
Assessing low-dose gentamicin-induced kidney injury in rats by analysis of urine: A. Nykjaer, et al.; J. Pharmacol. Toxicol. Methods 60, 316 (2009), Abstract;
Differences in immunolocalization of Kim-1, RPA-1, and RPA-2 in kidneys of gentamicin-, cisplatin-, and valproic acid-treated rats: potential role of iNOS and nitrotyrosine: J. Zhang, et al.; Toxicol. Pathol. 37, 629 (2009), Abstract;

General Literature References

Application of emerging biomarkers of acute kidney injury in development of kidney-sparing polypeptide-based antibiotics: D. Burt, et al.; Drug Chem. Toxicol. 37, 204 (2014), Abstract;

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