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Isopeptidase T (long form) (human), (recombinant)



BML-UW9690-0025	25 µg	185.00 USD
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Product Details

Alternative Name:	USP5, Ubiquitin-specific protease 5

MW:	~97 kDa

Source:	Produced in E. coli. Accession number: P45974, length 858 amino acids.

UniProt ID:	P45974-1

Formulation:	Liquid. In 50mM TRIS, 10mM β-mercaptoethanol, pH 7.5.

Purity:	≥90% (SDS-PAGE)

Shipping:	Dry Ice

Long Term Storage:	-80°C

Scientific Background:	Isopeptidase T is a ubiquitin-specific processing protease (UBP) that specifically disassembles free K48-linked polyubiquitin chains into ubiquitin monomers. There are two human isopeptidase T isoforms; Isopeptidase T short form (isoT-S) and Isopeptidase T long form (isoT-L) which differ by an insertion of 23 amino acids in isoT-L. Isopeptidase T is the only UBP known to require zinc binding to be active.

Regulatory Status:	RUO - Research Use Only

Isopeptidase T (long form) (human), (recombinant) SDS-PAGE

Fig 1: Coomassie stained gel of: recombinant human IsoT-L (UW9690) after SDS-PAGE [lane (a) showing molecular weight standards].

Isopeptidase T (long form) (human), (recombinant) AA

Fig 2: Deubiquitinylation assay for Isoeptidase-T (long form). Indicated quantities of Isopeptidase T (Prod # BML-UW9690) were added to 100 ul total volume containing 13 uM Ub-AMC (Prod # BML-SE211) in 50 mM HEPES pH 7.8, 0.5 mM EDTA, 0.1 mg/ml ovalbumin, 1 mM DTT. AMC release was measured by fluorescence (Ex 360 nm, Em 460 nm) and converted to weight using a standard curve of free AMC (Prod # BML-KI107).

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