Mature protein for functional studies.
Product Details
| Alternative Name: | Sentrin-3, Small ubiquitin-related modifier 3, SUMO-2 |
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| MW: | 15.5 kDa |
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| Source: | Produced in E. coli. Human SUMO-3 is fused to an N-terminal His-tag. |
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| UniProt ID: | P55854 |
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| Formulation: | Liquid. In 20mM HEPES, pH 8.0, containing 50mM sodium chloride and 1mM dithiothreitol. |
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| Purity: | ≥95% (SDS-PAGE) |
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| Quality Control: | Ability to SUMOlyate RanGAP substrate (Prod. No. BML-UW9755) in vitro. |
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| Application Notes: | Uses:
1. SUMO-3 modification of specific proteins in vitro.
2. Demonstrate novel proteins are potential targets for SUMOylation under in vitro conditions.
3. Generate substrates for deSUMOylating enzymes. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | Conjugation of mature SUMO proteins to specific lysine residues on target proteins via isopeptide bonds, is involved in a range of processes including nuclear protein targeting, formation of sub-nuclear complexes, regulation of transcriptional activities, and control of protein stability. |
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| Regulatory Status: | RUO - Research Use Only |
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Western Blot analysis: Lane 1: MW marker, Lane 2: SUMO-3 probed with SUMO-2/3 pAb.
SDS-PAGE analysis: Lane 1: MWM, Lane 2: 1μg SUMO-3 recombinant protein.
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Product Literature References
Resolving the zinc binding capacity of honey bee vitellogenin and locating its putative binding sites: V. Leipart, et al.; Insect Mol. Biol.
31, 810 (2022),
Abstract;
