Product Details
MW: | ~18kDa |
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Source: | Produced in E. coli. |
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UniProt ID: | P62837 |
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Formulation: | Liquid. In 25mM TRIS-HCl, pH 7.5, containing 150mM NaCl and 0.5mM DTT. |
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Purity: | ≥90% (SDS-PAGE) |
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Biological Activity: | The His6-tagged fusion proteins of UbcH5a, UbcH5b and UbcH5c all charge and support ubiquitinylation in vitro. Unlike their GST-tagged counterparts, the His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions. The [C85A] mutation (Prod. No. BML-UW9065) completely abolishes the ability of the enzyme to form a thiol ester and thereby acts as an excellent negative control. |
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Application Notes: | Useful for in vitro ubiquitinylation reactions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation, which can occur with GST-tagged versions. |
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Shipping: | Dry Ice |
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Long Term Storage: | -80°C |
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Use/Stability: | Enzyme is stable to multiple freeze/thaw cycles. |
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Scientific Background: | A number of E2s in Saccharomyces cerevisiae and their homologues have been identified. One such family of E2s is the UBC4/5, characterised as essential for the degradation of short-lived, regulatory and abnormal proteins. Protein levels of S. cerevisiae UbC4/5 are up-regulated in response to stress, and their loss results in severe effects on cellular functions.
A human gene product that is 79% identical to S. cerevisiae UBC4/5 in amino-acid sequence was identified as UbcH5a. In addition, two other human members of this highly conserved E2 class were also cloned and designated as UbcH5b and UbcH5c, having 88% and 89% identity to UbcH5a, respectively.
Members of the UbcH5a/b/c are the most active class of E2s in cell extracts. The importance of this enzyme class is underscored by the critical role of UBC4/5 in S. cerevisiae. UbcH5a stimulates the conjugation of ubiquitin to the tumour-repressor p53 in the presence of E6-AP and E6. Moreover, UbcH5 family is implicated in c-fos recognition, the modulation of which is controlled by the ubiquitin-proteasome pathway. UbcH5b and UbcH5c are associated with the signal-induced conjugation and subsequent degradation of IkBα in the presence of the SCF complexes. UbcH5c also catalyses the ubiquitination leading to the processing of p105 precursor to form p50, a subunit of the heterodimeric transcription factor NF-kB. The range and diversity of substrates and E3s with which this class of E2 enzymes interact, suggest their complex roles in cellular functions require to be studied further. |
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Regulatory Status: | RUO - Research Use Only |
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SDS-PAGE analysis: Lane 1: MW Marker, Lane 2: 1 µg UbcH5b (human), (recombinant) (His-tag).
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Product Literature References
Pepper E3 ligase CaAIRE1 promotes ABA sensitivity and drought tolerance by degradation of protein phosphatase CaAITP1: W. Baek, et al.; J. Exp. Bot.
138, 1093 (2021),
Abstract;
UBXN2A enhances CHIP-mediated proteasomal degradation of oncoprotein mortalin-2 in cancer cells: S. Sane, et al.; Mol. Oncol.
12, 1753 (2018),
Abstract;
Full Text
Modulating cellular balance of Rps3 mono-ubiquitination by both Hel2 E3 ligase and Ubp3 deubiquitinase regulates protein quality control: Y. Jung, et al.; Exp. Mol. Med.
49, e390 (2017),
Abstract;
Full Text
The Pepper RING-Type E3 Ligase CaAIRF1 Regulates ABA and Drought Signaling via CaADIP1 Protein Phosphatase Degradation: C.W. Lim, et al.; Plant Physiol.
173, 2323 (2017),
Abstract;
Full Text
Identification and functional expression of the pepper RING type E3 ligase, CaDTR1, involved in drought stress tolerance via ABA-mediated signalling: H. Joo, et al.; Sci. Rep.
6, 30097 (2016),
Application(s): In vitro ubiquitination,
Abstract;
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Loss of NEDD4 contributes to RTP801 elevation and neuron toxicity: implications for Parkinson's disease: M. Canal, et al.; Oncotarget
7, 58813 (2016),
Abstract;
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Regulation of the Abundance of Kaposi's Sarcoma-Associated Herpesvirus ORF50 Protein by Oncoprotein MDM2: T.H. Chang, et al.; PLoS Pathog.
12, e1005918 (2016),
Abstract;
Full Text
Comprehensive functional characterization of cancer-testis antigens defines obligate participation in multiple hallmarks of cancer: K.E. Maxfield, et al.; Nat. Commun.
6, 8840 (2015),
Application(s): In vitro autoubiquitination assays,
Abstract;
UBXN2A regulates nicotinic receptor degradation by modulating the E3 ligase activity of CHIP: Y. Teng, et al.; Biochem. Pharmacol.
97, 518 (2015),
Application(s): Ubiquitinylation,
Abstract;
General Literature References
Identification of the ubiquitin carrier proteins, E2s, involved in signal-induced conjugation and subsequent degradation of IkappaBalpha: H. Gonen et al.; J. Biol. Chem. 274, 14823 (1999),
Enzymes catalyzing ubiquitination and proteolytic processing of the p105 precursor of nuclear factor kappaB1. : Coux, O. and Goldberg, A.L.; J. Biol. Chem. 273, 8820-8 (1998),
Degradation of the proto-oncogene c-Fos by the ubiquitin proteolytic system in vivo and in vitro: identification and characterisation of the conjugating enzymes: I. Stancovski et al.; Mol. Cel. Biol. 15, 7106 (1995),
Identification of a family of closely related human ubiquitin conjugating enzymes: J.P. Jensen et al.; J. Biol. Chem. 270, 30408 (1995),
Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53. : Scheffner, M.; Proc. Natl. Acad. Sci. USA 91, 8797-8801 (1994),
The ubiquitin-conjugation system. : Jentsch, S. ; Annu. Rev. Genet. 22, 179-207 (1992),
Ubiquitin-conjugating enzymes UBC4 and UBC5 mediate selective degradation of short-lived and abnormal proteins. : Seufert, W. and Jentsch, S.; EMBO J. 9, 543-50 (1990),
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