Otubain-1 (human), (recombinant) (His-tag)



BML-UW0680-0100	100 µg	346.00 USD
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Product Details

Alternative Name:	OTUB1, Ubiquitin thioesterase OTUB1, Ubiquitin-specific-processing protease OTUB1, Deubiquitinating enzyme OTUB1

MW:	~33kDa

Source:	Produced in E. coli.

EC:	3.1.2.-

UniProt ID:	Q96FW1

Formulation:	Liquid. In 50mM TRIS, pH 7.5, containing 50mM NaCl and 1mM DTT.

Purity:	≥95% (SDS-PAGE)

Application Notes:	Uses: 1. Deubiquitinylation assays using polyubiquitin chains or ubiquitin-protein conjugates. 2. Studies of the K⁴⁸-linkage specific polyubiquitin chain binding and deubiquitinylation. 3. Investigation of the role of Otubain-1 in various biological processes. 4. Development of inhibitors for Otubain-1 deubiquitinylating activity.

Shipping:	Dry Ice

Short Term Storage:	-80°C

Long Term Storage:	-80°C

Use/Stability:	Stable for at least 6 months after receipt when stored at -80°C.

Handling:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.

Scientific Background:	Otubain-1 belongs to the ovarian tumor (OTU) domain class of cysteine protease deubiquitinating enzymes and has been implicated in mediating lymphocyte antigen responsiveness and may be generally involved in RNA processing and cell adhesion/morphology. Human otubain-1 has been shown to exhibit high linkage-specificity in vitro, cleaving Lys⁴⁸ (K⁴⁸)-linked polyubiquitin but not K⁶³-, K²⁹-, K⁶-, or K¹¹-linked polyubiquitin, or linear α-linked polyubiquitin. Cleavage is not limited to either end of a polyubiquitin chain, and both free and substrate-linked polyubiquitin are disassembled.

Regulatory Status:	RUO - Research Use Only

Otubain-1 (human), (recombinant) (His-tag) SDS-PAGE

Figure 1: SDS-PAGE image of His₆-Otubain-1 (Coomassie). Lane 1: MW markers. Lane 2: Otubain-1 (human) (recombinant) (His) (Prod. No. BML-UW0680) (8µg).

Otubain-1 (human), (recombinant) (His-tag) Western blot

Figure 2: Deubiquitinylation assay: Western Blot showing polyubiquitin chains (K⁴⁸-linked, Prod. No. BML-UW8860; K⁶³-linked, Prod. No. BML-UW9570) incubated with (+) or without (-) Otubain-1 (Prod. No. BML-UW0680), detected with ubiquitin polyclonal antibody (Prod. No. BML-UG9510) (1:1'000)"

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General Literature References

Evidence for bidentate substrate binding as the basis for the K48 linkage specificity of otubain 1: T. Wang, et al.; J. Mol. Biol. 386, 1011 (2009), Abstract;

Structural basis and specificity of human otubain 1-mediated deubiquitination: M.J. Edelmann, et al.; Biochem. J. 418, 379 (2009), Abstract;

Two isoforms of otubain 1 regulate T cell anergy via GRAIL: L. Soares, et al.; Nat. Immunol. 5, 45 (2004), Abstract;

Otubains: a new family of cysteine proteases in the ubiquitin pathway: M.Y. Balakirev, et al.; EMBO Rep. 4, 517 (2003), Abstract;