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[pSer¹⁸⁵]14-3-3 polyclonal antibody



BML-SA479-0025	25 µl	141.00 USD
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Replaces Prod. #: ALX-215-026

Product Details

Alternative Name:	KCIP-1, Protein kinase C inhibitor protein 1, 14-3-3β/ζ, Protein 1054

Host:	Sheep

Immunogen:	14-3-3 phosphorylated at Ser¹⁸⁵.

UniProt ID:	P31946 (human 14-3-3β), P63104 (human 14-3-3ζ)

Species reactivity:	Human, Mouse, Rat Bovine, Sheep

Specificity:	Recognizes 14-3-3β and 14-3-3ζ phosphorylated at Ser¹⁸⁵.

Applications:	WB

Recommended Dilutions/Conditions:	Western Blot (1:300-500) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Purity Detail:	Affinity purified phospho-specific antibody.

Shipping:	Dry Ice

Long Term Storage:	-80°C

Regulatory Status:	RUO - Research Use Only

[pSer185]14-3-3 polyclonal antibody Western blot

Western blot analysis performed with BML-SA479 antibody (1:500), secondary anti-sheep antibody (1:2’000), blotted against 2μg each of: 1. Recombinant 14-3-3β expressed in E.coli 2. Recombinant 14-3-3ζ expressed in E.coli 3. Sheep brain homogenate, which contains ~50% of β and ζ isoforms that are phosphorylated on Ser¹⁸⁵.

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Product Literature References

Increased 14-3-3 phosphorylation observed in Parkinson's disease reduces neuroprotective potential of 14-3-3 proteins: S.R. Slone, et al.; Neurobiol. Dis. 79, 1 (2015), Application(s): Western Blot, Abstract;

General Literature References

Novel brain 14-3-3 interacting proteins involved in neurodegenerative disease.: S. Mackie & A. Aitken; FEBS J. 272, 4202 (2005), Abstract;

Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein.: P. Steinacker et al.; Mol. Cell Biol. 25, 1339 (2005), Abstract;

Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1.: H-K. Chen et al.; Cell 113, 457 (2003), Abstract;

Mammalian and yeast 14-3-3 isoforms form distinct patterns of dimers in vivo.: M. Chaudhri et al.; Biochem. Biophys. Res. Commun. 300, 679 (2003), Abstract;

Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants.: A. Aitken; Plant Mol. Biol. 50, 993 (2002), Abstract;

Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain.: H.C. Baxter et al.; Neuroscience 109, 5 (2002), Abstract;

Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease.: J. Wiltfang et al.; J. Neurochem. 73, 2485 (1999), Abstract;

14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain.: M. Matto-Yelin et al.; Mol. Biol. Cell 8, 1889 (1997), Abstract;

The structural basis for 14-3-3:phosphopeptide binding specificity.: M.B. Yaffe et al.; Cell 91, 961 (1997), Abstract;

Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation.: C.P. Wheeler-Jones et al.; Biochem. J. 315, 41 (1996), Abstract;

Neurofibrillary tangles of Alzheimer’s disease brains contain 14-3-3 proteins.: R. Layfield et al.; Neurosci. Lett. 209, 57 (1996), Abstract;

14-3-3 alpha and delta are the phosphorylated forms of raf-activating 14-3-3 beta and zeta. In vivo stoichiometric phosphorylation in brain at a Ser-Pro-Glu-Lys MOTIF.: A. Aitken et al.; J. Biol. Chem. 270, 5706 (1995), Abstract;

Expression and structural analysis of 14-3-3 proteins.: D.H. Jones et al.; J. Mol. Biol. 245, 375 (1995), Abstract;

Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins.: D.H. Jones et al.; FEBS Lett. 368, 55 (1995), Abstract;

Purification of 14-3-3 protein and analysis of isoforms in chicken brain.: Y. Patel et al.; Biochim. Biophys. Acta. 1222, 405 (1994), Abstract;

Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies.: H. Martin et al.; J. Neurochem. 63, 2259 (1994), Abstract;

Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein.: H. Martin et al.; FEBS Lett. 331, 296 (1993), Abstract;