Product Details
| Alternative Name: | Hypoxia-inducible factor 1α, ARNT-interacting protein |
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| Clone: | H1α67 |
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| Host: | Mouse |
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| Isotype: | IgG |
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| Immunogen: | Recombinant human hypoxia-inducible factor-1α (HIF-1α) (aa 432-528). |
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| UniProt ID: | Q16665 |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Ferret, Monkey, Porcine, Rabbit, Sheep
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| Applications: | ELISA, Flow Cytometry, ICC, IF, IHC, IHC (FS), IHC (PS), IP, WB
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| Application Notes: | Detects a band of ~120kDa by Western blot. |
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| Purity Detail: | Purified IgG. |
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| Formulation: | Liquid. In PBS with 1% BSA containing 0.05% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry: HIF-1 alpha, mAb (H1alpha67) [Prod. no. BML-SA287] - Staining of HIF1 alpha in human kidney. Renal tubular epithelium showed moderate membranous, cytoplasmic and nuclear staining, and glomeruli showed faint to moderate nuclear staining.
Immunocytochemistry/Immunofluorescence: HIF-1 alpha, mAB (H1alpha67) [Prod. no. BML-SA287] - Expression of HIF-1 in interstitial pig cells under 12% of 02 condition.
Western Blot: HIF-1 alpha, mAb (H1alpha67) [Prod.no. BML-SA287] - Analysis of 50ug cobalt chloride (CoCl2) induced COS-7 nuclear extracts.
Western Blot: HIF-1 alpha, mAb (H1alpha67) [Prod.no BML-SA287] - HIF-1 alpha induction by CoCl2 on Caki-1 cell lysate.
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Product Literature References
Potential role of CXCR4 in trastuzumab resistance in breast cancer patients: R.M. Kotb, et al.; Biochim. Biophys. Acta Mol. Basis Dis.
1868, 166520 (2022),
Abstract;
