Replaces Prod. #: ALX-804-210
Product Details
| Alternative Name: | Poly(ADP-ribose) polymerase-1 |
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| Clone: | C-2-10 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Purified calf thymus poly(ADP-ribose) polymerase (PARP). |
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| UniProt ID: | P18493 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Hamster, Monkey
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| Specificity: | Recognizes an epitope in the C-terminal part of the DNA binding domain of PARP. |
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| Crossreactivity: | Does not cross-react with chicken PARP. |
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| Applications: | ELISA, ICC, WB
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| Recommended Dilutions/Conditions: | Western Blot (1µg/ml, alkaline phosphatase)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects bands ~116kDa (intact PARP) and ~85kDa (apoptosis-induced cleavage fragment) by Western blot. |
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| Purity Detail: | Affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -20°C |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of whole cell extracts from (1) human HL60 leukemia cells (Prod. No. BML-SW101) and (2) HL60 cells induced to undergo apoptosis using etoposide (Prod. No. BML-SW102). The nitrocellulose membrane was probed with purified clone C-2-10 mouse monoclonal anti-PARP (Prod. No. BML-SA249) at 1 µg/ml. Secondary antibody was GAM-AP (1/2000) and the nitrocellulose membrane was developed with BCIP/NBT. Arrows correspond to 116kDa and 85kDa.
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Product Literature References
Expression of BRCA1, BRCA2, RAD51, and other DSB repair factors is regulated by CRL4WDR70: Z. Mirman, et al.; DNA Repair (Amst.)
113, 103320 (2022),
Abstract;
New Insights into the Significance of PARP-1 Activation: Flow Cytometric Detection of Poly(ADP-Ribose) as a Marker of Bovine Intramammary Infection: G.D. Matteis, et al.; Cells
10, 599 (2021),
Abstract;
Combinatory Treatment of Canavanine and Arginine Deprivation Efficiently Targets Human Glioblastoma Cells via Pleiotropic Mechanisms: O. Karatsai, et al.; Cells
9, 2217 (2020),
Abstract;
Autophagy requires poly(adp-ribosyl)ation-dependent AMPK nuclear export: J.M. Rodríguez-Vargas, et al.; Cell Death Differ.
23, 2007 (2016),
Application(s): Western blot, MCF7 GFP-LC3 cells,
Abstract;
PRIMA-1(MET) induces death in soft-tissue sarcomas cell independent of p53: T. Grellety, et al.; BMC Cancer
15, 684 (2015),
Application(s): Immunoblot analysis,
Abstract;
Alzheimer's disease-associated polymorphisms in human OGG1 alter catalytic activity and sensitize cells to DNA damage: K.D. Jacob, et al.; Free Radic. Biol. Med.
63, 115 (2013),
Application(s): Western blot,
Abstract;
Regulation of poly(ADP-ribose) polymerase-1 functions by leukocyte elastase inhibitor/LEI-derived DNase II during caspase-independent apoptosis: C. Leprête, et al.; Int. J. Biochem. Cell Biol.
41, 1046 (2009),
Abstract;
CCCTC-binding Factor Activates PARP-1 Affecting DNA Methylation Machinery: T. Guastafierro, et al.; J. Biol. Chem.
283, 21873 (2008),
Abstract;
Full Text
Nuclear poly(ADP-ribose) polymerase-1 rapidly triggers mitochondrial dysfunction: G. Cipriani, et al.; J. Biol. Chem.
280, 17227 (2005),
Abstract;
Full Text
Inactivation of p21WAF1 sensitizes cells to apoptosis via an increase of both p14ARF and p53 levels and an alteration of the Bax/Bcl-2 ratio: D. Javelaud & F. Besançon; J. Biol. Chem.
277, 37949 (2002),
Abstract;
Full Text
Characterization of the necrotic cleavage of poly(ADP-ribose) polymerase (PARP-1): implication of lysosomal proteases: S. Gobeil, et al.; Cell Death Differ.
8, 588 (2001),
Abstract;
Selective loss of poly(ADP-ribose) and the 85-kDa fragment of poly(ADP- ribose) polymerase in nucleoli during alkylation-induced apoptosis of HeLa cells: R. Alvarez-Gonzalez, et al.; J. Biol. Chem.
274, 32122 (1999),
Abstract;
Full Text
Activation of a CrmA-insensitive, p35-sensitive pathway in ionizing radiation-induced apoptosis: R. Datta, et al.; J. Biol. Chem.
272, 1965 (1997),
Abstract;
Characterization of anti-peptide antibodies directed towards the automodification domain and apoptotic fragment of poly (ADP-ribose) polymerase: P.J. Duriez, et al.; Biochim. Biophys. Acta
1334, 65 (1997),
Abstract;
Different cleavage pattern for poly(ADP-ribose) polymerase during necrosis and apoptosis in HL-60 cells: G.M. Shah, et al.; Biochem. Biophys. Res. Commun.
229, 838 (1996),
Abstract;
FADD/MORT1 is a common mediator of CD95 (Fas/APO-1) and tumor necrosis factor receptor-induced apoptosis: A.M. Chinnaiyan, et al.; J. Biol. Chem.
271, 4961 (1996),
Abstract;
Full Text
Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution: Y.A. Lazebnik, et al.; PNAS
92, 9042 (1995),
Abstract;
Full Text
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature
371, 346 (1994),
Abstract;
Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis: S.H. Kaufmann, et al.; Cancer Res.
53, 3976 (1993),
Abstract;
Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study: D. Lamarre, et al.; Biochim. Biophys. Acta
950, 147 (1988),
Abstract;
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