Product Details
| Host: | Rabbit |
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| Immunogen: | Synthetic peptide corresponding to aa 399-415 (CE339QARWEAASKETIK412) of human parkin. A single amino acid substitution (A405E) exists in both the rat and mouse homologues. |
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| UniProt ID: | O60260 |
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| Species reactivity: | Human
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| Applications: | IHC, IP, WB
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| Blocking Peptide: | For Blocking peptide see Prod. No. BML-PP9365.
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| Formulation: | Liquid. In PBS. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Parkin is a ubiquitin-protein ligase (E3), and mutations in the Parkin gene appear to be closely associated with autosomal recessive juvenile Parkinson’s disease. Parkin has been localised to Lewy bodies in patients with sporadic Parkinson’s disease, and in peri-nuclear aggresome-like inclusions in cells over-expressing wild-type Parkin where proteasomal activity has also been inhibited4. Both types of inclusion also contain ubiquitin, α-synuclein, proteasomal subunits and heat shock proteins. A recent paper suggests that loss of proteasomal activity leads to Parkin accumulation with a concomitant reduction in E3 ligase activity resulting in Lewy body formation5.Parkin is selectively recruited from the cytosol to damaged mitochondria to trigger their autophagy (mitophagy). |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Methamphetamine oxidatively damages parkin and decreases the activity of 26S proteasome in vivo: A. Moszczynska, et al.; J. Neurochem.
116, 1005 (2011),
Application(s): Immunoprecipitation and Western blot,
Abstract;
Full Text
General Literature References
PINK1 Is Selectively Stabilized on Impaired Mitochondria to Activate Parkin: D.P. Narenda, et al.; PLoS Biol.
8, e1000298 (2010),
Abstract;
Parkin is recruited selectively to impaired mitochondria and promotes their autophagy: D. Narendra, et al.; J.Cell.Biol.
183, 795 (2008),
Abstract;
