Product Details
Alternative Name: | Proteasome subunit β type-7, Macropain chain Z |
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Clone: | MCP165 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Dinitrophenylated proteasomes. |
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UniProt ID: | Q99436 (human), P70195 (mouse) |
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Gene/Protein Identifier: | PSMB7 (gene name) |
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Source: | Purified from hybridoma tissue culture supernatant. |
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Species reactivity: | Human, Mouse
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Specificity: | Recognizes the β2 subunit of the 20S proteasome. |
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Applications: | IHC, WB
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Recommended Dilutions/Conditions: | Western Blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Immunoprecipitation: Does not immunoprecipitate intact proteasomes from HeLa cell preparations.
Immunohistochemistry: Has been shown to have applicability in immunohistochemical applications using human tissue.
Western blot: Single dimension SDS-PAGE of a HeLa cell lysate followed by Western blotting gives a single band with a relative molecular weight of ~28kDa. |
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Purity Detail: | Protein G affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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Use/Stability: | Store unopened vial at -20°C until required for use. Aliquot undiluted antibody into smaller volumes (not less than 10μl) prior to freezing if appropriate. The use of high quality ‘antiserum-grade’ plastic or glass vials is recommended. Store diluted antibody at 2-4°C (do not freeze) and use within 1 month. |
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Handling: | Avoid freeze/thaw cycles. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | The hybridoma secreting the antibody to subunit β2 was generated by fusion of splenocytes from Balb/c mice which had received repeated immunization with dinitrophenylated proteasomes. The antibody (clone MCP165) has been characterised by one- and two dimensional Western blotting. The antibody has not been demonstrated to immunoprecipitate the native 20S proteasome. The immunoglobulin subclass is IgG1. |
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Technical Info/Product Notes: | Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from "standard" nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins. |
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Regulatory Status: | RUO - Research Use Only |
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Western Blot Analysis of Proteasome 20S β2 subunit, mAb (MCP165), (Prod. No. BML-PW9300): Lane 1: MW Marker, Lane 2: HeLa Cell Lysate (Prod. No. ADI-LYC-HL100)
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Product Literature References
An SOD1 deficiency aggravates proteasome inhibitor bortezomib-induced testicular damage in mice: T. Homma & J. Fujii; Biochim. Biophys. Acta Gen. Subj.
1863, 1108 (2019),
Application(s): Mouse cryosectioned tissue,
Abstract;
SOD1 deficiency decreases proteasomal function, leading to the accumulation of ubiquitinated proteins in erythrocytes: T. Homma, et al.; Arch. Biochem. Biophys.
583, 65 (2015),
Application(s): Immunoblotting,
Abstract;
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