Product Details
| Alternative Name: | Proteasome subunit β type-6, Macropain δ chain, Proteasome subunit Y |
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| Clone: | MCP421 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Dinitrophenylated proteasomes. |
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| UniProt ID: | P28072 |
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| Gene/Protein Identifier: | PSMB6 (gene name) |
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| Source: | Purified from mouse ascites. |
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| Species reactivity: | Human
Rabbit, Yeast
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| Specificity: | Recognizes the β1 subunit of the 20S proteasome. |
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| Applications: | IHC, WB
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| Purity Detail: | Protein G-affinity purified ascites. |
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| Formulation: | Liquid. In PBS containing 10mM sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | Various systems for the nomenclature of the proteasome subunits have been established. This may be a source of confusion as the system on UniProt differs from "standard" nomenclature as described in the literature. The UniProt ID and Gene Name will help to clearly identify the proteins. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Proteasome 20S β1 subunit, mAb (MCP421) (Prod. No. BML-PW8140): Lane 1: MW marker, Lane 2: HeLa (Prod. No. ADI-LYC-HL100), Lane 3: HeLa S100 fraction (Prod. No. BML-SW8750), Lane 4: 3T3 (Prod. No. ADI-LYC-3T100).
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Product Literature References
Healthspan improvement and anti-aggregation effects induced by a marine-derived structural proteasome activator: M.A. Vasilopoulou, et al.; Redox Biol.
56, 10262 (2022),
Abstract;
Red Blood Cell Proteasome in Beta-Thalassemia Trait: Topology of Activity and Networking in Blood Bank Conditions: A.T. Anastasiadi, et al.; Membranes
11, 716 (2021),
Abstract;
An SOD1 deficiency aggravates proteasome inhibitor bortezomib-induced testicular damage in mice: T. Homma & J. Fujii; Biochim. Biophys. Acta Gen. Subj.
1863, 1108 (2019),
Application(s): Mouse cryosectioned tissue,
Abstract;
Novel cell line models to study mechanisms and overcoming strategies of proteasome inhibitor resistance in multiple myeloma: D. Brunnert, et al.; Biochim. Biophys. Acta Mol. Basis Dis.
1865, 1666 (2019),
Abstract;
Aging Triggers Cytoplasmic Depletion and Nuclear Translocation of the E3 Ligase Mahogunin: A Function for Ubiquitin in Neuronal Survival: S. Benvegnu, et al.; Mol. Cell
66, 358 (2017),
Abstract;
Detection of active proteasome structures in brain extracts: proteasome features of August rat brain with violations in monoamine metabolism: P.A. Erokhov, et al.; Oncotarget
8, 70695 (2017),
Abstract;
Full Text
Circadian modulation of proteasome activity and accumulation of oxidized protein in human embryonic kidney HEK 293 cells and primary dermal fibroblasts: A. Desvergne, et al.; Free Radic. Biol. Med.
94, 195 (2016),
Application(s): Western blot,
Abstract;
Skeletal muscle myotubes in severe obesity exhibit altered ubiquitin-proteasome and autophagic/lysosomal proteolytic flux: L.M. Bollinger, et al.; Obesity (Silver Spring)
23, 1185 (2015),
Application(s): Western Blot,
Abstract;
Full Text
SOD1 deficiency decreases proteasomal function, leading to the accumulation of ubiquitinated proteins in erythrocytes: T. Homma, et al.; Arch. Biochem. Biophys.
583, 65 (2015),
Application(s): Immunoblotting,
Abstract;
The GST-BHMT assay reveals a distinct mechanism underlying proteasome inhibition-induced macroautophagy in mammalian cells: Y.N. Rui, et al.; Autophagy
11, 812 (2015),
Abstract;
Full Text
Ubiquitin-independent proteosomal degradation of myelin basic protein contributes to development of neurodegenerative autoimmunity: A. Belogurov Jr, et al.; FASEB J.
29, 1901 (2015),
Abstract;
Full Text
The small heat shock protein B8 (HSPB8) confers resistance to bortezomib by promoting autophagic removal of misfolded proteins in multiple myeloma cells: M. Hamouda, et al.; Oncotarget
5, 6252 (2014),
Application(s): Analysis of velcade resistant multiple myeloma human cells by WB, Assay,
Abstract;
Full Text
Purification and separation of the 20S immunoproteasome from the constitutive proteasome and identification of the subunits by LC-MS: V. Dechavanne, et al.; Protein Exp. Purif.
87, 100 (2013),
Application(s): Western blot,
Abstract;
Bortezomib resistance in a myeloma cell line is associated to PSMβ5 overexpression and polyploidy: P. Balsas, et al.; Leuk. Res.
36, 212 (2012),
Application(s): Western blot,
Abstract;
TRIM5α associates with proteasomal subunits in cells while in complex with HIV-1 virions: Z. Lukic, et al.; Retrovirology
8, 93 (2011),
Abstract;
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