Product Details
| Clone: | P4D1 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Denatured bovine ubiquitin. |
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| UniProt ID: | P0CG53 (UBB), P0CH28 (UBC), P62992 (RPS27A), P63048 (UBA52) |
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| Source: | Purified from tissue culture supernatant. HRP conjugated chemically in vitro. |
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| Species reactivity: | Species independent
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| Specificity: | Recognizes mono- and poly-ubiquitin protein conjugates, free polyubiquitin chains and free ubiquitin. |
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| Applications: | WB
|
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Ion exchange purified. |
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| Formulation: | Liquid. In PBS containing 0.01% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice Not Frozen |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | +4°C |
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| Scientific Background: | Ubiquitin modification of cellular proteins has been shown to be involved with a wide range of biochemical processes including proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy. |
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| Technical Info/Product Notes: | P4D1 antibody was developed using a denatured glutaraldehyde cross-linked ubiquitin IgG complex as the immunogen. Monoclonal antibody was purified from tissue culture supernatant by ion exchange chromatography. The general ubiquitin reactivity of the monoclonal antibody has been demonstrated by Western blotting using synthetic ubiquitin chains, single isopeptide-linked polyubiquitin chains, and with endogenously ubiquitinylated species in cell lysates. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Inhibition of USP1 enhances anticancer drugs-induced cancer cell death through downregulation of survivin and miR-216a-5p-mediated upregulation of DR5: S.M. Woo, et al.; Cell Death Dis.
13, 821 (2022),
Abstract;
Activation of nuclear PTEN by inhibition of Notch signaling induces G2/M cell cycle arrest in gastric cancer: S.J. Kim, et al.; Oncogene
35, 251 (2016),
Application(s): Western Blot,
Abstract;
General Literature References
A role for ubiquitin in selective autophagy: V. Kirkin, et al.; Mol. Cell.
34, 259 (2009),
Abstract;
Proteasome-independent functions of ubiquitin in endocytosis and signaling: D. Mukhopadhyay & H. Riezman; Science
315, 201 (2007),
Abstract;
TAK1 is a ubiquitin-dependent kinase of MKK and IKK: C. Wang, et al.; Nature
412, 346 (2001),
Abstract;
DOT4 links silencing and cell growth in Saccharomyces cerevisiae: A. Kahana & D.E. Gottschling; Mol. Cell Biol.
19, 6608 (1999),
Abstract;
The ubiquitin-proteasome pathway: on protein death and cell life: A. Ciechanover; EMBO J.
17, 7151 (1998),
Abstract;
